XXXIInd Annual Clinical Cytometry Meeting and Course


Printable Program




October 6-10, 2017

Phoenix, Arizona






Friday, September 28, 2018


8:00 - 8:30 AMBallroom Foyer
COFFEE

8:30 - 12:00 PMGrand Ballroom II
FRIDAY COURSE AM

8:30
Welcome
Andrea Marcogliese
Texas Children's Hospital

8:45
Introduction To Clinical Flow Cytometry
Fiona Craig
Mayo Clinic Arizona

9:45
Instrumentation And Sample Preparation
Paul Wallace
Roswell Park

10:45
Coffee Break

11:00
Normal Findings In Hematolymphoid Tissues
Sunita Park
Children's Healthcare of Atlanta

1:30 - 5:00 PMGrand Ballroom II
FRIDAY COURSE PM

1:30
B-Cell Lymphoid Neoplasms Part 1
Andrea Marcogliese
Texas Children's Hospital

2:30
B-Cell Lymphoid Neoplasms Part 2
Adam Seegmiller
Vanderbilt University

3:30
Coffee Break

4:00
T/Nk Cell Lymphomas
Kristy Wolniak
Northwestern University

6:00 - 7:30 PMBallroom Foyer
COURSE WELCOME RECEPTION



Saturday, September 29, 2018


7:30 - 8:00 AMBallroom Foyer
COFFEE

8:00 - 12:15 PMGrand Ballroom II
SATURDAY COURSE AM

8:00
Standardization
Teri Oldaker
Independent Consultant

9:00
Controlling All Steps Of Flow Cytometry Assays
Andrea Illingworth
Dahl-Chase Diagnostic Services

10:00
Coffee

10:15
Acute Lymphoblastic Leukemia
Michael Borowitz
Johns Hopkins Medicine

11:15
Acute Myeloid Leukemia
Sindhu Cherian
University of Washington

1:45 - 5:30 PMGrand Ballroom II
ADVANCED GROUP: LECTURES

1:45
Chronic Myeloid Stem Cell Neoplasms
Sa Wang
MD Anderson

2:45
Minimal Residual Disease Testing
Brent Wood
University of Washington

3:45
Coffee Break

4:15
Case Studies In Lymphoid Neoplasia: Diagnostic Dilemmas And Lessons Learned
Steve Kussick
Pheno Path

5:15
Q&A

1:45 - 5:30 PMGalleria I
BEGINNER GROUP: COMPUTER LAB

1:45
Introduction To Listmode Analysis And Pattern Recognition
Michael Borowitz
Johns Hopkins Medicine

3:45
Coffee Break

4:15
Introduction To Listmode Analysis And Pattern Recognition Cont.
Michael Borowitz
Johns Hopkins Medicine



Sunday, September 30, 2018


7:30 - 8:00 AMBallroom Foyer
COFFEE

8:00 - 12:00 PMGrand Ballroom II
SUNDAY COURSE AM

8:00
Primary Immunodeficiency
Michele Paessler
Children's Hospital of Philadelphia

8:45
Acquired Immunodeficiency
Jolene Cardinali
Hartford Healthcare

9:30
Coffee

9:45
Plasma Cell Neoplasms
Maryalice Stetler-Stevenson
NIH

10:45
Case Studies In Validation And Quality
Andrea Illingworth1, Teri Oldaker2
1Dahl-Chase Diagnostic Services, 2Independent Consultant

11:45
Wrap Up
Andrea Marcogliese
Texas Children's Hospital

12:00 - 2:00 PMGalleria II & III
CORPORATE LUNCH & LEARN

Chair(s): Mike Linden
12:00
Bd Biosciences

12:30
Beckman Coulter

1:00
Fluidigm

1:30
Sysmex

2:15 - 5:00 PMGrand Ballroom II
PLENARY SESSION 1: FRONTIERS IN FLOW CYTOMETRY

Chair(s): Sindhu Cherian
2:15
Welcome
Mike Keeney
London Health Sciences Centre

2:30
Data-Driven Cytometry: Updates In Automated Data Analysis
Ryan Brinkman
British Columbia Cancer Agency

3:10
Fish Flow In Cll
Kathy Fuller
University of Western Australia

3:50
Coffee Break - Sponsored By Bangs Laboratories Inc

4:20
Sorting Of Placental Extracellular Vessicles: Techniques And Potential Applications
Terry Morgan
Oregon Health & Science University

5:00 - 5:40 PMGrand Ballroom II
WALLACE H. COULTER LECTURE

Chair(s): Mike Keeney
5:00
Wallace H. Coulter Award Lecture
Paul Wallace
Roswell Park

5:45 - 7:15 PMGrand Ballroom I
WELCOME RECEPTION/ POSTER SESSION 1

P1
Digital Poster: Comprehensive Polychromatic Flow Cytometry Profiling Of The Healthy Human Immune System
Jeffrey T. Guptill1, Marilyn Rosa-Bray2, Janet Staats3, Pearl Zakroysky1, Cliburn Chan4, John S. Yi3, Scott White3, Kent J. Weinhold3
1Duke Clinical Research Institute, Durham, NC, United States, 2Biomat USA – Grifols Plasma Operations, Seattle, WA, United States, 3Department of Surgery, Duke University Medical Center, Durham, NC, United States, 4Department of Biostatistics, Duke University School of Medicine, Durham, NC, United States

P3
Digital Poster: Introducing New Technologies For Immune Monitoring In Drug Trials:  30-Parameter Flow Cytometry, Phenoscans, And Cytobrute Applied To A Melanoma Immunotherapy Trial
Pratip K Chattopadhyay1, Santi Adavani2, Vinay Rao2, Aidan Winters1
1Precision Immunology, NYU Langone Health, New York, NY, United States, 2RocketML, Portland, OR, United States

P5
Flow Cytometry Assay Validation As A Biomarker Compared To A Drug Develpment Setting
Audrey Gagnon, Dominic Gagnon, Jean-Francois Poulin, Bader Yassine-Diab, Martin Lelaidier, Virginia Litwin
Caprion Biosciences, Montreal, QC, Canada

P7
A Novel Storage Media For Preserving Cerebrospinal Fluid (Csf) Cells For Flow Cytometry
Alka A Redkar, Mariel Shockley, Sunita I Park
Children's Healthcare of Atlanta (CHOA), Atlanta, GA, United States

P9
Eight Color Flow Cytometry Panels For The Evaluation Of Lymphocyte Subsets Using Streck Controls
Alexander J Vogel, Alex L Lloyd, Carissa R Moore
Streck Inc, La Vista, NE, United States

P11
Evaluation Of Blast Cell Percentage In Myelodysplastic Syndrome By Flow Cytometry 
Benjamin D Hedley1, Mary Xie2, Nikhil Sangle1, Cyrus Hsia3, Janice Popma1, Mike Keeney1, Ian Chin-Yee3
1Department of Pathology and Laboratory Medicine, London Health Sciences Centre, London , ON, Canada, 2Department of Pathology and Laboratory Medicine, Western University, London , ON, Canada, 3Department of Medicine, Division of Hematology, London Health Sciences Centre, London , ON, Canada

P13
Clinical Laboratory Validation Of Platelet Surface Glycoprotein Assessment By Flow Cytometry
Gregory E. Otteson1, Constance P. Chen2, Rajiv K. Pruthi1, Rong He1, Deepti M. Warad1, Min Shi1, Dong Chen1
1Mayo Clinic, Rochester, MN, United States, 2Mayo High School, Rochesgter, MN, United States

P15
Clearllab 10C Panels Performance: Specimen And Reagent Carryover And Panel Lot-To-Lot Reproducibility
Li Yang, Casey L Roberts, Jin Zhang, Lidice Lopez
Beckman Coulter, Miami, FL, United States

P17
Efficiency Of Leukocyte Differential By Flow Cytometry With Cytodiff In Different Workflows
Chenxue Qu, Jianzhong Wang, Chengwei Pu, Ying Xing
Peking University Fiirst Hospital, Beijing, China

P19
Utility Of Flow Cytometry Based Platelet Aggregation Testing In Patients With Suspected Platelet Function Defect
Praveen Sharma1, Jasmina Ahluwalia1, Man U S Sachdeva1, Narender Kumar1, Neelam Varma1, Sunil Bose1, Parveen Bose1, Deepak Bansal2, Pankaj Malhotra3
1Department of Hematology, Post Graduate Institute of Medical Education and Research , Chandigarh, India, 2Department of Pediatrics, Post Graduate Institute of Medical Education and Research , Chandigarh, India, 3Department of Internal Medicine, Post Graduate Institute of Medical Education and Research , Chandigarh, India

P21
Development And Implementation Of A Flow Cytometry Assay To Measure Receptor Occupancy Of An Anti-Cd73 Antibody To Target In A Clinical Trial
Ed Hilt1, Preeti Yamdagni1, Uma Purandare1, Todd Rogers2, Lisa Patti-Diaz1, Yongliang Sun1, Anke Klippel1, Nathan Hedrick1
1Bristol-Myers Squibb, Princeton, NJ, United States, 2Q2 Solutions, Atlanta, GA, United States

P23
Demonstration Of Clinical And Controls Precision Of The Clearllab 10C Panels In A Multi-Center Study 
Astrid Smit1, Guoyan Cheng1, Mike Keeney2, Joanne Luider3, Wolfgang Kern4, Adrian Padurean5, Liliana Tejidor1, Robert Magari1
1Clinical Research, Beckman Coulter, Inc., Miami, FL, United States, 2London Health Sciences Center, London, ON, Canada, 3Calgary Laboratory Services, Calgary, AB, Canada, 4MLL Munich Leukemia Laboratory, Munich, Germany, 5Neogenomics Laboratory, Inc, Fort Myers, FL, United States

P25
Utility Of Flow Cytometry In The Initial Diagnosis Of Myeloproliferative Neoplasms
Hanna Chang, Rina E. Eden, Lilia G. Sanchez, David T. Lynch
Brooke Army Medical Center, San Antonio, TX, United States

P27
Variables Contributing To Inability Of Flow Cytometry To Detect Lymphoma: A Retrospective Study 
Madison E. Hannay, Alisa M. Caudell, Tiffany G. Baker, Michael Chajewski, Olga S. Chajewski, Angie Duong, Peter M. Houston, Jessica S. Snider, Jack Yang, Kathryn G. Lindsey
Medical University of South Carolina , Charleston, SC, United States

P29
Evaluation Of Tim-3 Expression For Receptor Occupancy Assay
Hua-Chen Chang, Melissa Tinsley, Lisa Green, Paul C Trampont
Translational Biomarker Solutions, Covance Greenfield Laboratories, Greenfield, IN, United States

P31
Clearllab 10C Panels Performance: Method Comparison To Predicate, Clearllab Reagents For Immunophenotyping Of Leukemia And Lymphoma
Jin Zhang, Han Gao, Jessica Jopek Ashbaugh, Lidice Lopez
Beckman Coulter, Inc., Miami, FL, United States

P33
Just In Time Flow Cytometry
Jinfeng Suo1, Daniel Martinez1, Lienmei Liao1, Clifford Blieden1, Mike Perez1, Christine Roth2
1Baylor St Luke's Medical Center, Houston, TX, United States, 2Baylor College of Medicine, Houston, TX, United States

P35
Inter-Laboratory Comparison Of The Transfix®/Edta Vacuum Blood Collection Tube With The 5 Ml Cyto-Chex® Bct
Daniel Harrison1, Rosalie Ward2, Sarah Bastow3, Andrew Parr3, Susan Macro4, Paul Wallace4
1Caltag Medsystems Limited, Buckingham, United Kingdom, 2Royal Hallamshire Hospital, Sheffield, United Kingdom, 3Royal Sussex County Hospital, Brighton, United Kingdom, 4Roswell Park Cancer Institute, Buffalo, NY, United States

P37
Lymphocyte Subset As A Reference Range In Healthy Saudi Population
MANAL S. ALSAYEGH1, ABDULLAH A. ALAMER4, MAHER KHOSYFAN3, FAISAL M. ALSERAYE2
1Security Forces Hospital, Riyadh, Saudi Arabia, 2Security Forces Hospital, Riyadh, Saudi Arabia, 3Security Forces Hospital, Riyadh, Saudi Arabia, 4Security Forces Hospital, Riyadh, Saudi Arabia

P39
Immunophenotyping Cd8+ Tscm Cells
Patrick E Kudlacek, Anamica Muruganandham, Curtis Sheldon, Rafiqul Islam
Celerion, Inc., Lincoln, NE, United States

P41
Validating The Use Of Transfix® To Stabilise Cerebrospinal Fluid (Csf) For Flow Cytometry Immunophenotyping Of Haematological Malignancies
Tim Almond1, Daniel Harrison1, M Hughes2, Michelle Crawford2, Ulrika Johansson2
1Caltag Medsystems Limited, Buckingham, United Kingdom, 2Bristol Royal Infirmary, Bristol, United Kingdom

P43
Single-Platform Cd34+/Cd45+ Cell Analysis On The Bd Facslyric™System Using Cap Samples
Yang Zeng, Angela Chen, Farzad Oreizy, Harshada Rohamare, Kevin Judge
BD Life Sciences, San Jose, CA, United States

P45
Comparison Of A Dual Platform Analysis Of Cd34+ Hematopoietic Stem Cell Enumeration On The Facscanto Ii And Facslyric Flow Cytometers
Yuvaporn Sookpisal1, Susan Atwater2, Jean Oak2
1Stanford Health Care, Stanford, CA, United States, 2Stanford University Department of Pathology, Stanford, CA, United States

P47
Cd8-Positive Anaplastic Large Cell Lymphoma 
Jing Shen, Shaoying Li, Pei Lin, C.Cameron Yin, Guilin Tang, Jeffrey L. Jorgensen, Sa A. Wang, Jie Xu
MD Anderson Cancer Center, Houston, TX, United States

P49
Detection Of Ribosomal Rna 5.8S By Intracellular Flow Cytometry And Immunohistochemistry As A Novel Marker Of Activation In Hematopoiesis
Marisa Juntilla, Jyoti Kumar, Eric Windt, Steve Artandi
Stanford University, Palo Alto, CA, United States

P51
Diagnostic Utility Of Cerebrospinal Fluid Flow Cytometry In Patients With Hematologic Malignancies
Horatiu Olteanu1, Bjorn Batdorf2
1Mayo Clinic, Rochester, MN, United States, 2Medical College of Wisconsin, Milwaukee, WI, United States

P53
Immunophenotypic Profiles Of Primary Mediastinal (Thymic) Large B-Cell Lymphoma, A Study Of 13 Cases
Suyang Hao1, Pei Lin2
1The Methodist Hospital, Houston, TX, United States, 2MD Anderson Cancer Center, Houston, TX, United States

P55
Preservation Of Intracellular Signaling In Aml Blood Samples For Clinical Correlative Studies
Sarah E Nataraj, Monica L Guzman
Weill Cornell Medicine, New York, NY, United States

P57
The Significance Of Tumor Infiltrating T Cells In Myc Rearranged Diffuse Large B Cell Lymphoma: A Flow Cytometry Study
Shaoying Li, Tahani Alhalouli, Sa Wang, Jeffrey Jorgensen, Pei Lin
UT MD Anderson Cancer Center, Houston, TX, United States

P59
Multicolor Flow Cytometry Reveals Clonal Nature Of Mast Cells In Patients Of Hematological Neoplasm With Mast Cell Proliferation
Sweta Rajpal1, Man Updesh Singh Sachdeva1, Anu Aggarwal1, Parveen Bose1, Reena Das1, Neelam Varma1, Pankaj Malhotra2
1Department of hematology, Post graduate institute of medical education and research, Chandigarh, India, 2Department of internal medicine, clinical hematology division, Post graduate institute of medical education and research , Chandigarh, India

P61
Validation And Implementation Of Dry Tube Technology In Flow Cytometry To Improve Efficiency And Increase Capacity
K Pacella1, S Gould1, B DuChateau2
1Health Network Laboratories, Allentown, PA, United States, 2NECAPS, Charlestown, RI, United States

P63
Improving Mrd Sensitivity Of Multiparameter Flow Cytometry With A Novel Enrichment Technology
Silin Sa1, Liping Yu1, Charlene&Bush Donovan1, Marisa Juntilla1, Jean Oak2, Suraj Saksena1
1BD Biosciences, San Jose, CA, United States, 2Stanford Uniersity, Stanford, CA, United States

P65
Validation Of A Single Tube For Minimal Residual Disease Detection Of B Lymphoblastic Leukemia On The Bd Facslyric 
Katelyn Dannheim, Erdyni Tsitsikov, Olga K. Weinberg
1, Boston, MA, United States

P67
Air-Dried Peripheral Blood And Bone Marrow As Reference And Control Materials
Yanli Liu1, Daniel Santos1, Andrew Smith1, Yuming Tang1, Menilou Franzblau1, Paul K. Wallace2, Joseph Tario2, Derek Jones3, Richard Schretzenmair3, Jonni Moore3, Arianna Gatti4, Bruno Brando4, Elena Afonina1, Marybeth Sharkey1
1BD Biosciences, San Jose, CA, United States, 2Roswell Park Comprehensive Cancer Center, NY, NY, United States, 3University of Pennsylvania, PA, PA, United States, 4Legnano General Hospital, Milano, Italy

P69
Stabilization Of Whole Blood With Germaben Ii (Gii) For Proficiency Testing Of Cd4/Cd8 Percentage And Absolute Counts
Raul Louzao1, John Wong1, Sylvia Hood1, Wes Rountree1, Marek Poniewierski1, Tracy Taylor2, Margot Plews2, Tamsir Diallo2, Adrienne Meyers2, Thomas Denny1
1Duke University, Duke Human Vaccine Institute, Durham, NC, United States, 2National HIV and Retrovirology Laboratory, Winnipeg, MB, Canada

P71
Quantitative Expression Of Monocytic Markers In Acute Myeloblastic Leukemia Cases To Differentiate Between Acute Monocytic Leukemias And Other Acute Myeloblastic Leukemia Subtypes
Sarah M. Mohamed1, Nahla M. Farahat1, Hanaa M. Donia1, Eman A. Nadwan2
1Alexandria University, Alexandria, Egypt, 2Haematology department, faculty of medicine, Alexandria University, Alexandria, Egypt

P73
Community Flow Lab Cog B-All Mrd Accreditation: You Can Do It!
Jennifer Stumph, Robert Humes, Juli Allaire, Susan Padilla, Pamela Kidd
Spectrum Health, Grand Rapids, MI, United States

7:15 - 8:15 PMGalleria II & III
WOMEN IN ICCS



Monday, October 1, 2018


8:00 - 8:30 AMGrand Ballroom I
COFFEE

8:15 - 8:30 AMGrand Ballroom II
BUSINESS MEETING

Chair(s): Mike Keeney
8:30 - 12:00 PMGrand Ballroom II
PLENARY SESSION 2: MYELOID NEOPLASMS: PRESENT AND FUTURE

Chair(s): Misha Roshal
8:30
Csi Case
Arjan van de Loosdrecht
VU University Medical Center

8:50
Best Paper Award: Reproducible Diagnosis Of Cll
Andrew Rawstron
HMDS Leeds, UK

9:10
Integrating Mrd Assessment By Flow Cytometry Into Clinical Management In Aml-Minimal Residual Disease In Aml: Consensus Document From Eln Mrd Working Party
Roland Walter
University of Washington

10:00
Coffee Break - Sponsored By Alexion

10:30
Flow Cytometry Versus Genetics For Mrd Assessment, When, What, And Why
Yi Zhou
University of Washington

11:15
Flow Cytometric Evaluation Of Mds In The Context Of Genetic Findings
Wolfgang Kern
MLL Münchner Leukämielabor GmbH

12:00 - 1:30 PMGalleria I
LUNCHEON WORKSHOP 2: INSTRUMENT SIMULATORS

12:00
Instrument Simulators-Practical Approach To Compensation Part 1
Bruce Greig
Vanderbilt University

12:00
Instrument Simulators-Practical Approach To Compensation Part 2
Amr Rajab
Lifelabs

12:00 - 1:30 PMGalleria II
LUNCHEON WORKSHOP 1: AML MRD DONE TWO WAYS

12:00
Aml Mrd Done Two Ways - Strategies Used In Two Different Laboratories Part 1
Sa Wang
MD Anderson

12:00
Aml Mrd Done Two Ways - Strategies Used In Two Different Laboratories Part 2
Yi Zhou
University of Washington

12:00 - 1:30 PMGalleria III
LUNCHEON WORKSHOP 4: PANEL DESIGN

12:00
Panel Design In The Era Of Anti-Antigen Therapy (Myeloma, Lymphoma, Leukemia) Part 1
Jonathan Fromm
University of Washington

12:00
Panel Design In The Era Of Anti-Antigen Therapy (Myeloma, Lymphoma, Leukemia)  Part 2
Maryalice Stetler-Stevenson
NIH

12:00 - 1:30 PMForum Suite
LUNCHEON WORKSHOP 3: ACCESSIBLE MACHINE LEARNING

Accessible Machine Learning For Clinical Flow Cytometry: New Semi Automated Approaches In Flow Cytometry Analysis 
Marie Christine Bene
CHU Nantes France

1:30 - 5:00 PMGrand Ballroom II
PLENARY SESSION 3: THE ROLE OF FLOW CYTOMETRY IN DRUG DEVELOPMENT

Chair(s): Virginia Litwin
1:30
Csi Case
Corey Allard
University of Iowa

1:50
The Perfect Match: Cellular Biomarkers Demonstrated How Apds Met Its Therapy
Ulrike Sommers
Novartis

2:35
Advancing Transformational Therapies In Hematologic Malignancies Through Novel Flow Cytometry Biomarkers
Cherie Green
Genentech

3:20
Coffee Break - Sponsored By Streck

3:50
Integrating Clinical Diagnosis And Biopharma Support In A Single Flow Cytometry Laboratory: Lessons Learned
Steve Kussick
Pheno Path

4:35
Round Table Qa Session: All Speakers

5:00 - 6:00 PMGrand Ballroom I
POSTER SESSION II

P12
Validation Models For Monitoring Bispecific Car T Cell Therapy In Acute Lymphoblastic Leukemia (All)
Linda S. Weaver1, Nirali N. Shah2, Terry J. Fry3, Haiying Qin2, Maryalice Stetler-Stevenson1
1Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States, 2Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States, 3University of Colorado School of Medicine, Denver, CO, United States

P14
Flow Cytometric Analysis Of Cerebrospinal Fluid: An Institutional Review And Practice Modification
Nina P Rahimi1, Melanie H Hakar1, Heidi Rahn2, Laura Marszalek1, Kristy L Wolniak1
1Northwestern University Feinberg School of Medicine, Chicago, IL, United States, 2NorthShore University HealthSystem, Evanston, IL, United States

P16
Screening B-Cell Lymphoproliferative Disorders (B-Lpd) Using One-Tube 10-Color, 15 Antibody Flow Cytometry (Fc) Panel And 6-Parameter Kaluza Radar Plot (Rp).
Amr Rajab, Afaf Erfaei, Miranda Wozniak
Medical–Scientific Department, LifeLabs Medical Laboratory Services, Toronto, ON, Canada

P18
Clearllab 10C Panels Performance: Specimens Processed Using Different Wash Methods And Collected In K2Edta, Heparin, And Acd Anticoagulants Demonstrate Equivalent Performance 
Rebecca J Ortiz Marty, Ileana Munoz Antoni, Jessica Ashbaugh, Jin Zhang, Lidice Lopez
Beckman Coulter, Miami, FL, United States

P20
Addition Of Cd71 To High-Sensitivity Cd235A/Cd59 Pnh Rbc Assay Allows The Additional Accurate Delineation Of Pnh Type Iii, Pnh Type Ii And Normal (Type I) Nucleated Rbcs
D. Robert Sutherland1, Stephen J Richards2, Fernando Ortiz3, Rakesh Nayyar4, Miro Benko5, Andrea Illingworth6
1University of Toronto, Toronto, ON, Canada, 2St James University Hospital, Leeds, United Kingdom, 3Ottawa Hospital Research Institute, Ottawa, ON, Canada, 4Cytoquest Corp., Toronto, ON, Canada, 5EXBIO, Prague, Czech Republic, 6Dahl-Chase Diagnostic Services, Bangor, ME, United States

P22
Making Sense Of A B Cell Marker On T Cells: Validation Of A Flow Cytometric Method Measuring Cd20 Expression On T Cell Subsets 
SRIRAM RAMANAN1, JOLENE KER2, NITHIANANDAN SELLIAH1, CHRISTELE GONNEAU3, KIKO LY2, AJAY GROVER1, BRAHMANANDA CHITTETI1, ELAINE LOURENCO1, TONY FAZIO3, SHUZK CHENG LOH2, LEANNE FLYE-BLAKEMORE4, ALAN LACKEY4, PAUL TRAMPONT5, YOAV PERETZ1
1HEMATOLOGY/FLOW CYTOMETRY, COVANCE CENTRAL LABORATORY SERVICES, INDIANAPOLIS, IN, United States, 2HEMATOLOGY/FLOW CYTOMETRY, COVANCE CENTRAL LABORATORY SERVICES, SINGAPORE, Singapore, 3HEMATOLOGY/FLOW CYTOMETRY, COVANCE CENTRAL LABORATORY SERVICES, GENEVA, Switzerland, 4COVANCE CENTRAL VALIDATION SERVICES, BRENTWOOD, TN, United States, 5TRANSLATIONAL BIOMARKER SOLUTIONS, COVANCE GREENFIELD LABORATORIES, GREENFIELD, IN, United States

P24
Development And Clinical Implementation Of Flow Cytometry Based Assay To Measure Tigit Receptor Occupancy (Ro) On T-Cell Sub Sets.   
Urmila Purandare1, Ed Hilt1, Lisa Patti-Diaz1, Karen Quadrini2, Thomas W Mc Closkey2, Nathan Hedrick1
1Bristol Myers Squibb Company (BMS), Pennington, NJ, United States, 2ICON Laboratory Services, Inc (ILS), Farmingdale, NY, United States

P26
Quantitation Of Neutrophilic Cd64 And Monocytic Hla-Dr Biomarkers Using Bd Quantibrite™ Reagents*
Wei Huang1, Chip Lomas1, Noel Warner1, Kevin Judge1, Paresh Jain2, Suraj Saksena1
1BD Biosciences, San Jose, CA, United States, 2BD Biosciences, San Jose, India

P28
Assessment Of Monocytes, Mdscs And Myeloid Cell Populations In Immuno-Oncology Clinical Trials By A Standardized High Complexity Flow Cytometry Approach
Zeni Alfonso1, Jane Gao1, Ghanashyam Sarikonda1, Nadia Katkova1, Christine Vaupel1, Ahmad Tarhini2, Naveen Dakappagari1
1Navigate Biopharma Services, Inc., A Novartis Subsidiary, Carlsbad, CA, United States, 2Cleveland Clinic Taussig Cancer Institute, Cleveland, OH, United States

P30
Clearllab Control Cells: A Process Control For Clearllab 10C Application
Li Yang, Samantha Bauchiero, Brittany Kuhl, Xiangyang Dong, Jin Zhang, Lilly Lopez
Beckman Coulter, Inc. Life Sciences, Research and Development, Miami, FL, United States

P32
Lymphocyte Immunophenotyping On The Bd Facslyric™System Using Cap Samples
Yang Zeng, Farzad Oreizy, Angela Chen, Harshada Rohamare, Kevin Judge
BD Life Sciences, San Jose, CA, United States

P34
Lymphocyte Proliferation In Vitro In Response To Tetanus And Candida Antigens By Flow Cytometry: Validation Of Major Test Parameters For The Clinical Laboratory
Eugene Ravkov1, Bruce Jolly1, Harry Hill1, 2, Eszter Lazar-Molnar1, 2
1ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, United States, 2Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, United States

P36
High Complexity All-In-One Flow Cytometry Assay For The Measurement Of T-Cell Activation, Checkpoint Inhibitor Expression And Proliferation Of T-Cells In Periphery As A Biomarker For Novel Immuno-Oncology Clinical Trials
Jennifer Tsau1, Ghanashyam Sarikonda1, Michael Abadier1, Brittney Atzmiller1, Christine Vaupel1, Ahmad Tarhini2, Naveen Dakappagari1
1Navigate BioPharma Services, Inc., A Novartis Subsidiary, Carlsbad, CA, United States, 2Cleveland Clinic Taussig Cancer Institute, Cleveland, OH, United States

P38
Flow Cytometry Features Of Incidental, Indolent T Lymphoblastic Proliferations
Jonathan R Fromm, Kerstin L Edlefsen, Sindhu Cherian, Lori Soma, Brent L Wood, David Wu
University of Washington, Seattle, WA, United States

P40
Rationale And Feasibility Of Using A Standardized Screening Tube On The Facscanto Ii And The Facslyric
Jean Oak, Jason Kurzer
Stanford University Department of Pathology, Stanford, CA, United States

P44
Identification Of Two Distinct Neoplastic Subpopulations By Flow Cytometry In A Patient With Ebv-Positive B-Cell Lymphoma And History Of Angioimmunoblastic T-Cell Lymphoma And T(8;21) Acute Myeloid Leukemia
Betty M Chung, April A Ewton, Arthur W Zieske
Houston Methodist Hospital, Houston, TX, United States

P46
Relapsed Cd19 Negative B-All Discovered Through Immune Status Monitoring
Amanda McCaustland, Tim Casey, Yoav Messinger, Gabriela Gheorghe
Children’s Minnesota Hospital, Minneapolis, MN, United States

P48
Cd177 Enhances The Detection Of Myelodysplastic Syndromes By Flow Cytometry
Howard Meyerson1, Jeremy Meyerson2, Samir Turakhia1
1University Hospital Cleveland Medical Center, Cleveland, OH, United States, 2Ohio State University, Columbus, OH, United States

P50
Acute Promyelocytic Leukemia Mimicking Paroxysmal Nocturnal Hemoglobinuria
I.J. Frame, Weina Chen, Franklin Fuda, Mingyi Chen
UT Southwestern Medical Center, Department of Pathology, Dallas, TX, United States

P52
Validation Of 8-Color Immunophenotyping Panels For Mature Lymphoid Neoplasms On The Bd Facslyric
Jyoti Kumar1, Yuvaporn Sookpisal2, Susan Atwater1, Jean Oak1
1Stanford University Department of Pathology, Stanford, CA, United States, 2Stanford Health Care, Stanford, CA, United States

P54
Streamlined Human Immune Monitoring With Mass Cytometry: 29 Markers In A Single Tube With Automated Data Analysis
Greg Stelzer1, Christina Loh1, Thiru Selvanantham1, Leslie Fung1, Michelle M Poulin1, C Bruce Bagwell2, Margaret Inokuma2, Clare E Rogers1, Steven T Lott1
1Fluidigm, South San Francisco, CA, United States, 2Verity Software House, Topsham, ME, United States

P56
Performance Of Bd Horizon Dri Leukocytes With Selected Antibody Panels
Daiva Gladding, Yuming Tang, Menilou Franzblau, Yanli Liu, Andrew Smith, Daniel Santos, Dennis van Hoof, Marybeth Sharkey, Elena Afonina
BD Biosciences, San Jose, CA, United States

P58
Metal Isotope-Tagged Monoclonal Antibodies For High-Dimensional Mass Cytometry
Guojun Han1, Garry Nolan2
1Stanford University, Stanford, CA, United States, 2Stanford University, Stanford, CA, United States

P60
Lean Study Comparing Manual And Automated Sample Preparation Methods Using The Investigational Bd Facscduet Integrated To Bd Facslyric™ System*. 
Alan Wu1, Cassadra Yun1, Imelda Omana-Zapata2, Lori Apoll3
1San Francisco General Hospital & Trauma Center, San Francisco, CA, United States, 2BD Biosciences, San Jose, CA, United States, 3BD Canada, Toronto, ON, Canada

P62
The Importance Of Being Regulated
Miroslav Benko, Aleš Efenberk, Hana Tichovská, Andrea Benešová, Pavel Jinoch, Miloslav Suchanek
EXBIO Praha a.s., Vestec, Czech Republic

P64
Sporadic But Specific Staining Artifact Involving The Apc-H7 Tandem Fluorochrome: Interpretive Challenges And Possible Mechanism
Catherine Luedke1, Holly N. Brown2, Beverly Williams2, Kathryn Perkinson2, Anand S. Lagoo1
1Department of Pathology, Duke University Medical Center, Durham, NC, United States, 2Duke University Health System Clinical Laboratories, Durham, NC, United States

P66
Treatment With Azacitidine Among Myelodysplastic Syndrome Patients Can Alter Cd33 Expression On Myeloid Progenitor Cells
Dana J Paine1, Laura M Pardo1,2, Denise A Wells1, Lisa E Brodersen1, Michael R Loken1
1Hematologics, Inc, Seattle, WA, United States, 2Fred Hutchinson Cancer Research Center, Seattle, WA, United States

P68
B-Cell Assay Portability Using The Bd Facslyric™ System. 
Jeffrey Jorgensen1, Than Nguyen1, Maurice O'Gorman2, Ruba Hse2, Carla Bocian3, Caren Mutschmann3
1Department of Hematopathology of the MD Anderson Cancer Cente, Huston, TX, United States, 2Synlab Analytics & Services Germany GmbH, Berlin, Germany, 3Keck School of Medicine, USC Chief of Laboratory Medicine Children's Hospital of Los Angeles, Los Angeles, CA, United States

P70
A Journey Through Implementation Of A New Laboratory Information System
Terri D Lebo1, Michael Batten2, Lindsay Holley1, Kari Caldwell1, Billy Moua1, Vijaya Knight3
1National Jewish Health, Denver, CO, United States, 2SCC Soft, Clearwater, FL, United States, 3Children's Hospital of Colorado, Aurora, CO, United States

P72
Neuroblastoma Detection By Flow Cytometry: A Rapid And Sensitive Approach
Jennifer Stumph, Robert Humes, Juli Allaire
Spectrum Health, Grand Rapids, MI, United States

P74
Patient-Specific Immunophenotypic Aberrant Profiles Observed During Longitudinal Clinical Trials
Damien Montamat-Sicotte, Dominic Gagnon, Josiane Chagnon-Choquet, Vicky Sgouroudis, Philippe Pouliot, Virginia Litwin
Caprion Biosciences Inc., Montreal, QC, Canada

P76
Immunophenotyping Using T-Sne: Analysis Of Acute Leukemia And Non-Disease Cases Tested Using Clearllab 10C Panels
Jin Zhang1, Benoit Dupont2, Casey L Roberts1, Lidice Lopez1
1Beckman Coulter, Miami, FL, United States, 2Beckman Coulter Marseille, Villepinte, France

P2
Digital Poster: Cd33 Expression By Natural Killer (Nk) Cells Is A Potential Confounder For Minimal Residual Disease Detection By Flow Cytometry
Ashley Eckel, Sindhu Cherian, Lorinda Soma
Department of Laboratory Medicine, University of Washington, Seattle, WA, United States

P4
Digital Poster: Clinical Outcomes In Mixed Phenotype Acute Leukemia (Mpal); A Multicenter Retrospective Cohort Study
Matthew Oberley1, Gerald Wertheim2, Sunil Raikar3, Richard Sposto1, Karen Rabin4, Joy Punia4, Alix Seif2, Reuven Schore5, Dragos Luca5, Terri Guinipero6, William Woods3, Etan Orgel1, Maurice O'Gorman1
1Children's Hospital Los Angeles, Los Angeles, CA, United States, 2Children's Hospital of Philadelphia, Philadelphia, PA, United States, 3Children's Hospital of Atlanta, Atlanta, GA, United States, 4Texas Children's Hospital, Houston, TX, United States, 5Children's National Health System, Washington, DC, United States, 6Nationwide Children's, Columbus, OH, United States

P6
Demonstration Of The Clinical Performance Of The Clearllab 10C Panels In A Multi-Center Study 
Astrid Smit1, Guoyan Cheng1, Mike Keeney2, Joanne Luider3, Wolfgang Kern4, Adrian Padurean5, Liliana Tejidor1, Robert Magari1
1Clinical Research, Beckman Coulter, Inc., Miami, FL, United States, 2London Health Sciences Center , London, ON, Canada, 3Calgary Laboratory Services, Calgary, AB, Canada, 4MLL Munich Leukemia Laboratory, Munich, Germany, 5Neogenomics Laboratory, Inc, Fort Myers, FL, United States

P8
T-, B- And Nk-Cell Enumeration Using The Investigational Automated Bd Facsduet Integrated To Bd Facslyric System
Lydia L. Lui, Josh Zollet, Beverly Lu, Imelda Omana-Zapata
BD Biosciences, San Jose, CA, United States

P10
Multi-Color Flow Cytometry For The Assessment Of Phenotypic And Functional Markers Associated With T-Cell Exhaustion. 
Suraj Saksena2, Mirko Corselli1, Stephanie Widmann2
1BD Biosciences, San Diego, CA, United States, 2BD Biosciences, San Jose, CA, United States

7:00 - 9:00 PMOff-site
BANQUET DINNER



Tuesday, October 2, 2018


7:00 - 8:00 AMGalleria II & III
CAP BREAKFAST WORKSHOP: CAP FLOW CYTOMETRY SURVEY CHALLENGES - COMMON AND UNCOMMON PITFALLS

Chair(s): William Karlon, Jolene Cardinali
8:00 - 8:30 AMGrand Ballroom I
COFFEE

8:30 - 11:30 AMGrand Ballroom II
PLENARY SESSION 4: REGULATORY ISSUES IN FLOW CYTOMETRY

Chair(s): Mike Linden
8:30
Csi Case
Hao-Wei Wang
NCI/NIH

8:50
Flow Cytometry When The Technical And Professional Components Are Performed In Different Laboratories: Perspectives Of The Laboratory And The Community Pathologist Part 1
Claire Murphy
Summit Pathology

9:10
Flow Cytometry When The Technical And Professional Components Are Performed In Different Laboratories: Perspectives Of The Laboratory And The Community Pathologist Part 2
David Ng
PhenoPath

9:30
New Clsi Document For The Validation Of Methods Preformed By Flow Cytometry-Sneak Peek And Update
Virginia Litwin
Caprion

10:10
Coffee Break

10:40
Standardization Of B-All Mrd: Standardization Of Interpretation, External Qa, Fda Approval
Mike Keeney
London Helath Sciences Centre

11:20
Q&A

11:30 - 1:00 PMGalleria I
LUNCHEON WORKSHOP 6: AML MRD

Aml Mrd: A Practical Approach (Computer Based Lab)
Sindhu Cherian
University of Washington

11:30 - 1:00 PMGalleria II
LUNCHEON WORKSHOP 5: GATING AND HISTOGRAM

11:30
Approaches To Gating And Histogram Setup Part 1
Adam Seegmiller
Vanderbilt University

11:30
Approaches To Gating And Histogram Setup Part 2
Aaron Shaver
Vanderbilt University

11:30 - 1:00 PMGalleria III
LUNCHEON WORKSHOP 7: VALIDATE A FLOW CYTOMETRIC ASSAY

11:30
How Do You Validate A Flow Cytometric Assay Focused The Detection Of Rare Events? Important Considerations For Rare Event Analysis And Assay-Specific Validation (Using The Example Of Pnh And Mrd In Multiple Myeloma) Part 1
Andrea Illingworth
Dahl-Chase Diagnostic Services

11:30
How Do You Validate A Flow Cytometric Assay Focused The Detection Of Rare Events? Important Considerations For Rare Event Analysis And Assay-Specific Validation (Using The Example Of Pnh And Mrd In Multiple Myeloma) Part 2
Misha Roshal
Memorial Sloan Kettering Cancer Center

11:30 - 1:00 PMForum Suite
LUNCHEON WORKSHOP 8: EVALUATING MDS AND CMML

11:30
The Approach To Evaluating Mds And Cmml By Flow Cytometry Part 1
Wolfgang Kern
MLL Münchner Leukämielabor GmbH

11:30
The Approach To Evaluating Mds And Cmml By Flow Cytometry Part 2
Arjan van de Loosdrecht
VU University Medical Center

1:00 - 3:30 PMGrand Ballroom II
LAVIA PARKER PLENARY SESSION: REACTIVE AND NEOPLASTIC T AND NK CELL POPULATIONS

Chair(s): Mike Keeney
1:00
Csi Case
Laura Marszalek
Northwestern Medicine

1:20
Regulatory T Cells In Malignancies
Troy Torgerson
Seattle Children's Hospital

2:00
Large Granular Lymphocytes, Reactive And Neoplastic Proliferations
Kristy Wolniak
Northwestern University

2:40
Ctcl Guidelines
Fiona Craig
Mayo Clinic Arizona

3:20
Q&A / Meeting Closing Remarks