Corporate Tutorials

Sunday, October 12th

8:30am-12:30pm

Location: 4th Floor in the Fifth Avenue Room

 

8:30 AM - 9:00 AM
Beckman Coulter

Title: CNTRP Initiative to Standardize Flow Cytometric Analyses of Patient Samples for Research Labs Across Canada

Speaker: Sabine Ivison, PhD.

Description: Canadian National Transplant Research Program (CNTRP):  Immune Monitoring Committee
Flow cytometry-based analysis of peripheral blood enables a better understanding of post-transplant immune status and response to therapy. The power and resolution of observations can be potentiated by combining data obtained at different sites. Seamless merging of data requires consensus markers for definition of cell populations as well as stringent standardization of panel architecture, staining protocols and cytometer settings. Groups like the European ONE Study have created a set of standardized panels and protocols for monitoring basic immune populations. In collaboration with Beckman-Coulter and the ONE Study, the CNTRP has extended this standardization to five research and clinical sites across Canada and included new panels to monitor cell populations implicated in transplantation tolerance.


9:00 AM - 9:30 AM

BD Biosciences

Title: Strategeis for Successful Multicolor Flow Cytometry Panel Design (Part 1)

Speaker: Bob Balderas, BD Biosciences

Description: With the introduction of new flow cytometry platforms, and the availability of fluorochromes such as the polymer dyes based on Sirigen technology and BD Horizon™ APC-R700, the development of 8–10-color applications has become easily accessible to basic and clinical researchers.

To ensure high quality of the information obtained from these assays, careful panel design is a critical step. First, we will discuss how panel design is an iterative process that is driven by profound knowledge of the biology, in terms of antigen level of expression and co-expression for every marker to be used in a specific panel. Then, we will review the information about fluorochrome resolution and spillover that is required. Finally, we will describe the correct analysis and interpretation of data obtained using the full multicolor panel, as well as data from all Fluorescence Minus One (FMO) controls, which are mandatory to accurately evaluate panel performance.

Following systematic recommendations and a workflow for panel design will ensure accurate and high- quality data.


9:30 AM - 10:00 AM
BD Biosciences

Title: Strategeis for Successful Multicolor Flow Cytometry Panel Design (Part 2)

Speaker: Bob Balderas, BD Biosciences

Description: With the introduction of new flow cytometry platforms, and the availability of fluorochromes such as the polymer dyes based on Sirigen technology and BD Horizon™ APC-R700, the development of 8–10-color applications has become easily accessible to basic and clinical researchers.

To ensure high quality of the information obtained from these assays, careful panel design is a critical step. First, we will discuss how panel design is an iterative process that is driven by profound knowledge of the biology, in terms of antigen level of expression and co-expression for every marker to be used in a specific panel. Then, we will review the information about fluorochrome resolution and spillover that is required. Finally, we will describe the correct analysis and interpretation of data obtained using the full multicolor panel, as well as data from all Fluorescence Minus One (FMO) controls, which are mandatory to accurately evaluate panel performance.

Following systematic recommendations and a workflow for panel design will ensure accurate and high- quality data.


10:00 AM - 10:30 AM
Alexion

Title: Recent Developments in Proficient PNH Flow Cytometry Testing

Speaker: Robert Sutherland, MD, PhD

Description: PNH is a debilitating blood disorder in which chronic, uncontrolled activation of complement, a component of the normal immune system, results in hemolysis (destruction of red blood cells), leading to blood clots, organ failure and shortened survival. This presentation will provide an update on the most recent advances in flow cytometry testing for PNH, most notably, utilizing CD157.


10:30 AM - 11:00 AM
De Novo Software

Title: What's new in FCS Express Version 5

Speaker: David Novo

Description: FCS Express IVD is the leading 3rd party flow cytometry data analysis tool for the clinical lab. This presentation will showcase the new features and improvements in FCS Express Version 5. Version 5 continues our tradition of combining an easy to use interface with best in class functionality and speed.

 

11:00 AM - 11:30 AM
Verity Software House

Title: New Developments in Analytical Software from Verity Software House

Speaker: Beth Hil, PhD

Description:  Compensation of flow cytometry data has been one of the biggest technical issues and problems since the beginning of flow cytometry. It is still one of the most talked about subjects on the Purdue flow cytometry list-service and has only become more complicated with the ability to collect higher-dimensional multi-parameter data. Verity Software House is introducing our new V-COMP™ feature; a fully-automated compensation system that is efficient, accurate, and easy to use for any number of parameters. As part of the new WinList 3D 8.0, V-COMP™ is compatible with any acquisition software currently in use.

We at Verity have also been busy with GemStone™ updates and applications. Looking for a program to analyze your CyTOF data in a reproducible and easily interpretable manner? Let us show you how GemStone™ does it without introducing the subjectivity of gating. GemStone is also busy counting StemCells at Roswell Park Cancer Institute and calculating the CD64 index for Trillium Diagnostics’ Leuko64 sepsis diagnostic kit. If you are looking to make your clinical lab more efficient and consistent with analysis, GemStone can help. 

ModFit LT™ continues to be the gold standard for DNA, Cell Cycle and Cell Proliferation analysis software.  With advanced features that allow for ploidy-based cell cycle analysis, drug-induced perturbation of cell cycle, complicated proliferation analysis with or without the presence of internal standards as well as cell tracking dye analysis, ModFit LT 4.0 uses wizards and batch processing to make your cytometry analysis automatic, fast, smooth and convenient for any number of files. Linked with WinList 3D, you can also include additional parameters to look at cell cycle proteins or other markers of interest.

Come see what ModFit™, GemStone™, and WinList™ can do for you; because without Verity, it’s just software.

 

11:30 AM - 12:00 PM
NeoGenomics Laboratories

Attendies: Kyle Dunn and Kristen Finholm

NeoGenomics, Inc. is a high-complexity CLIA—certified clinical laboratory that specializes in cancer genetics diagnostic testing, the fastest growing segment of the laboratory industry.  The company's testing services include cytogenetics, fluorescence in-situ hybridization (FISH), flow cytometry, immunohistochemistry, anatomic pathology and molecular genetic testing.  Headquartered in Fort Myers, FL, NeoGenomics has labs in Nashville, TN, Irvine, CA, Tampa, FL and Fort Myers, FL.  NeoGenomics services the needs of pathologists, oncologists, other clinicians and hospitals throughout the United States.

 

12:00 PM - 12:30 PM
Miltenyi Biotec GmbH

Title:  Flow sorting without droplets: Imagine a sterile, aerosol-free, high speed sorter.

Speaker: John Foster, President and Founder of Owl Biomedical, Inc.

As researchers face growing demands sorting cells, reducing operator risk, maintaining sample sterility and increasing the ease-of-use have become additional values sought by researchers. Miltenyi Biotec has in partnership with OWL Biomedical developed the all new MACSQuant Tyto, the first closed sorter system on the market. By using a sterile, one-way cartridge system that enables droplet and aerosol free measurements, the risk of contamination of the sample or the operator is successfully eliminated.  The MACSQuant Tyto sorts the sample in a disposable cartridge that incorporates a patented microchip (MEMS) and the world’s fastest valve using 3 lasers and 8 fluorescent parameters. After running the sample the positive and negative cell populations can be easily recovered for subsequent analysis or processing for downstream applications, e.g. cell processing using the CliniMACS® Prodigy.  The system is fully featured with an on board temperature control using Peltier solid state system, and a convenient, compact space saving design. The MACSQuant Tyto is designed to accommodate everyday experiments amongst clinical researchers with minimal training and ideal for all core facilities, clinical research labs and production facilities.


 

 

 
 
International Clinical Cytometry Society
2111 Chestnut Ave - Suite 145
Glenview, IL 60025 USA
 
847-550-3080
info@cytometry.org