Quality & Standards

The ICCS Quality and Standards committee is dedicated to the optimization of fundamental flow cytometric testing components. Its purpose is to identify major areas of variability, determine critical components needing standardization, develop and define acceptability standards and criteria, and provide guidance and measures for practical implementation in the laboratory. This group will work closely with the ICCS education committee and other entities as necessary.

The Q&S committee is comprised of 4 groups (instrument optimization, reagents and panels, specimen preparation and reporting) which will address the most common areas of variability in flow cytometry. The information will be presented in peer-reviewed “modules” with the goal to provide the laboratory staff with a practical reference guide in optimizing their procedures.

Module #1

Lysing Methods and Reagents for Flow cytometric Immunophenotyping
By Melanie O’Donahue, MT, ASCP, CCy and Laura Johnson, MT, ASCP, SM, SH

There is no consensus in the flow cytometry industry on which method of lysing erythrocytes is optimal. Different protocols might be more appropriate in different situations and differences in specimen preparation are a potential source of variability regarding the final results. In this first ICCS Quality and Standards module two experienced flow cytometry technologists present their findings about how the most commonly used lysing reagents may impact the quality of the results. We encourage you to email us at info@cytometry.org with any questions you might have regarding this module.