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| Educational Lecture Series |
The ICCS Education Committee announces the addition of a new section to the ICCS web site. This section contains contributions by experts in their respective areas on topics of interest to the clinical flow cytometry community. The presentations are based on slides from previous talks the authors have given, but have been edited to contain additional explanatory information, usually in a notes section, so that the education information in each presentation stands on its own. In addition, all have been peer reviewed. They are made available at no charge for personal use by ICCS membership with the understanding that credit will be given to the contributing author.
To kick off this educational series, 7 web talks have been placed on the site representing both introductory and advanced topics. These are:
- Introduction to Flow Cytometry, Philip McCoy, Jr., PhD, leads off with a comprehensive explanation of how conventional and some non-conventional flow cytometers work. He also covers experimental design, some non-traditional assays and finishes with guidelines about publishing your data.
- Compensation: It’s not just for pretty pictures, Mario Roederer, PhD, discuses how to correctly compensate high level multicolor experiments, how to detect improperly compensated data along with the use of biexponential data transformations. He also details pitfalls commonly encountered, proper staining controls for compensation and the use of FMO controls.
- Flow Cytometry for Acute Myeloid Leukemia, by Sindhu Cherian, MD, discuses in her contribution the use of flow cytometry in the diagnosis and management of acute myeloid leukemia as performed in her lab using up to 10 color panels. Gating techniques, examples of differentiation patterns and abnormal antigen expression are illustrated. She also covers the use of flow cytometry in detection of minimal residual disease.
- T cell Lymphoma Analysis, by Charles Goolsby, PhD, illustrates different aberrant T cell antigens patterns in mycosis fungoides, sézary syndrome, adult T cell leukemia/lymphoma (ATLL), T lymphoblastic lymphoma/leukemia, gamma/delta T cell lymphoma, and angioimmunoblastic T cell lymphoma. He also discussed the use of Vβ and KIR analysis in the diagnosis of clonality.
- Flow Cytometric Analysis of CSF Involvement of Leukemia or Lymphoma, Maryalice Stetler-Stevenson, MD, PhD, describes the use of flow cytometry for the detection of CSF involvement particularly for B cell non-Hodgkins lymphoma and compares flows sensitivity to cytomorphology. Sample requirements, stabilization techniques, protocols and antibody panels are also covered.
- Quality Control and Assessment in Flow Cytometry, David Barnett, PhD and Teri Oldaker, cover in their contribution internal QC and QA systems, external quality assessment, and the training and competency assessment of clinical flow cytometry staff.
Additional presentations currently in development will address instrument performance and quality assessment, multiparameter data analysis, CD34 enumeration, B cell neoplasms, acute lymphocytic leukemia, and ZAP70 quantification in chronic lymphocytic leukemia. The ICCS Education Committee welcomes your comments on those posted to date and suggestions for future topics.
Paul Wallace, PhD
Roswell Park Cancer Institute, Department of Flow and Image Cytometry.
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