Estimation of Fetomaternal Haemorrhage using a Flow Cytometric Technique
The anti-D flow cytometric technique has been employed within our laboratory since April 2007 as a replacement for the ‘Kleihauer-Betke’ acid elution method.  Over 3,500 screens have been performed and quantitations have been performed in 31 cases where the bleed exceeded 2ml. Of these, 22 cases (0.61%) had bleeds greater than 4 mls.
Rosalie Y Ward, Sheffield Teaching Hospitals Foundation Trust, Sheffield, UK.

Laboratory and Clinical Practice for Monitoring Sepsis with Neutrophil CD64 Index 
Prior to 2005, Cincinnati Children’s Hospital Medical Center physicians in the Division of Neonatology relied primarily on clinical judgment, white blood cell counts and differential, and blood cultures to evaluate potentially infected infants.  However, this approach was failing to identify a significant portion of infected infants and promoted the use of excessive antibiotics in uninfected infants.  Therefore, we set out to identify an improved method. 
Daniel Marmer and Paul Kingma Cincinnati Children’s Hospital Medical Center, OH, USA.

Flow Cytometry in the Hematology Lab:  The Flow Cytometry WBC Differential
Our group recently investigated an 8-color, single-tubce, lyse, no-wash flow cytometric method to perform an extended 8 part differential as a potential replacement reference method for WBC differential.  This method has many potential applications including validation of new hematology automated analyzers and clarifying cases with difficult morphology. 
Sindhu Cherian, University of Washington, Seattle, WA, USA.

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