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Conference Program


The ICCS 2020 Virtual Meeting program will be created from both live events and pre-recorded (on-demand) events.

The live events will take place from September 28-30, 2020 and will be recorded. Pre-recorded material will be available on-demand to view at a time which is convenient to you. All meeting materials will be available to view until October 31, 2020.

Times listed below are based on Eastern Time.


  Mon - Sep 28      Tue - Sep 29      Wed - Sep 30   


Monday, September 28th

10:00 - 12:15 PM
WELCOME & PLENARY SESSION 1: SARS-COV2/COVID-19
Chair/Organizer: Sindhu Cherian, Virginia Litwin

The SARS-CoV2/COVID-19 pandemic has had a profound impact not only on global health but also on our individual lives at work, in the laboratory, and at home. Since the initial descriptions in December 2019 of this novel viral agent and the disease it causes, the number of cases reported has skyrocketed with over 18 million cases documented worldwide as of August 4, 2020 (https://coronavirus.jhu.edu/map.html). Clinical cytometry provides a critical tool for characterizing the immune response to SARS-CoV2/COVID-19. In this session we bring our ICCS audience state of the art information delivered by accomplished speakers with experience on the front lines of this pandemic.


10:15
A Fireside Chat with William A. Haseltine: Perspective on the Global Pandemic
William Haseltine.

10:30
Q&A
.

10:55
What Flow Cytometry can Contribute to Understanding a Pandemic
Andrea Cossarizza.

11:30
Q&A
.

11:35
Updates in Vaccines and Therapy for SARS-CoV2/COVID-19
Jean Boyer.

12:10
Q&A
.
12:45 - 1:45 PM
EDUCATIONAL WORKSHOP 1: BASIC DATA ANALYSIS - CONSIDERATIONS IN TEMPLATE DESIGN

This workshop will go over illustrative examples of basic concepts of developing assay-specific analysis templates for clinical flow cytometry panels designed to investigate varied hematolymphoid disorders including PNH and acute leukemia with intra-cellular staining, among others. The scope of this presentation will include live analysis, when feasible, using panels and analysis software from each of the participating panelists and focus on designing the optimal plots and utilizing the appropriate gating strategy for each assay. 


12:45
Educational Workshop 1: Presentation 1
Andrea Illingworth.

12:55
Educational Workshop 1: Presentation 2
George Deeb.

1:010
Educational Workshop 1: Presentation 3
David Ng.

1:20
Educational Workshop 1: Presentation 4
Wolfgang Kern.

EDUCATIONAL WORKSHOP 2: PATTERNS OF MATURATION - DISTINGUISHING MATURE FROM IMMATURE CELLS

Maturation of hematopoietic cells is characterized by conserved changes in antigen expression that accompany progression from an immature or progenitor form to a mature effector cell.  In this workshop we will review antigenic changes that accompany B and T lymphoid maturatation.  Then, we will evaluate a series of cases that provide classificaition challenges due to immunophenotypic alterations that make determination of maturational stage challenging. Objectives for this workshop are as follows: • Identify patterns of antigen expression that characterize normal B cell and T cell maturation • Describe immunophenotypic features that distinguish immature and mature lymphoid cells • Distinguish neoplastic B cells and T cells from background normal cells • Characterize neoplastic cells as mature or immature • Recognize diagnostic challenges that may confound assessment of maturation stage


Patterns of Maturation - Distinguishing Mature from Immature Cells
Sindhu Cherian.

EDUCATIONAL WORKSHOP 3: TECHNICAL ASPECTS OF INSTRUMENT SET-UP

Flow cytometry has an essential role in the diagnosis and monitoring of hematopoietic neoplasms. Increased sophistication means that flow cytometry is now readily performed with 8-10+ antibodies in a single tube. This workshop will address technical aspects of instrument setup that are required to resolve cellular populations consistently and discriminate positive and negative marker expression for quantifying and determining marker co-expression. These settings should be consistent allowing for data reproducibility day to day, over time, and across instruments.


12:45
Educational Workshop 3: Presentation 1
Ben Hedley.

1:15
Educational Workshop 3: Presentation 2
Abby Kelliher.

EDUCATIONAL WORKSHOP 4: WHAT IS IN THE BACKGROUND? IDENTIFYING AND ANALYZING UNCOMMON CELLS

This presentation will focus on  highlighting neoplsms of uncommon cell populations in the bone marrow using case examples. Discussion will also include immunophenotypic features of normal NK cells, plasmacytoid dendritic cells and basophils.


12:45
Educational Workshop 4: Presentation 1
Franklin Fuda.

1:00
Educational Workshop 4: Presentation 2
Sanam Loghavi.
2:15 - 3:30 PM
AWARD PRESENTATIONS & WALLACE H. COULTER LECTURE
Chair/Organizer: Sindhu Cherian

Each year the ICCS presents the Wallace H. Coulter Award to an individual from anywhere in the world, recognizing his or her lifetime contributions to the science, education and practice of Clinical Cytometry. This prestigious award carries with it an honorarium, and the awardee presents the Keynote lecture at the annual Clinical Cytometry meeting.


Build Your Own Cytometrist
Fiona Craig.
3:30 - 4:30 PM
VIRTUAL POSTER WALK
Chair/Organizer: Adam Seegmiller

The virtual poster walk will showcase the top poster presentations and give attendees the opportunity to ask questions live to these presenters.


3:30
Welcome
.

3:35
Single antibody evaluation of T-cell receptor beta chain monotypia by flow cytometry facilitates the diagnosis of T-cell large granular lymphocytic leukemia
Min Shi.

3:40
Q&A
.

3:50
Screening B-cell Populations using a Single Bivariate Pattern Recognition Dot Plot (CD20 vs. CD38)
Amr Rajab.

3:55
Q&A
.

4:00
A New Method to Detect 24 Cytokines in Peripheral Blood Post Chimeric Antigen Receptor-T Cell Therapy by Flow Cytometry and Microbeads
Dongchu Wang.

4:05
Q&A
.

4:15
One Tube 24-colors Panel for Spectral Multicolor Flow Cytometry can Establish a Normal Myeloid Maturing and Expression Database of Bone Marrow
Hui Wang.

4:20
Q&A
.
5:00 - 5:30 PM
MONDAY NETWORKING SESSIONS

This is an opportunity to meet new people and learn more about various topics. Topics will include first time attendees, the education committee, trainees (residents/fellows/students), social media in flow cytometry, global networking (Asia/Australia/Pacific/Central & South America), and career advancements of technologists and global networking

6:00 - 7:30 PM
WOMEN IN CYTOMETRY
Chair/Organizer: Lama Kdouh, Julie Qualtieri

This is a live interactive session and attendance is limited to 200 people. Sign in early to make sure you get a spot! If you are not able to join in, rest assured that the content delivered by Dr. Hunt will be available for viewing on demand after the session.

We kindly ask you to take a moment to complete the Women in Cytometry survey: Click here to take the survey


Building self-confidence by overcoming imposter syndrome
Jennifer Hunt.
7:30 - 8:30 PM
ON DEMAND: VIRTUAL POSTERS

1
Single-platform assay for the enumeration of CD34+ cells with CD3+ drop-in option for the BD FACSLyric System
Ahmad Al-Attar. University of Kentucky, Lexington, KY, USA

2
Development and validation of an ISHAGE Guidelines Acquisition and Analysis template using FASCSuiteTMsoftware for the FACSLyricTMCytometer.
Ahmad Al-Attar1, Fernando Ortiz2, Rakesh Nayyar2, Ian Taylor3, D. Robert Sutherland4. 1University of Kentucky, Lexington, KY, USA.2Cytoquest Corporation, Toronto, ON, Canada.3Cytoquest Corporation, Toronto, ON, Canada.4BD Life Sciences, Ashland, OR, USA.5University of Toronto, Toronto, ON, Canada

3
A multiparameter flow cytometry assay to monitor megakaryocytes and leukemic cells in clinical trials of myelofibrosis patients
Zeni Alfonso1, Coral Zhao1, Anil Pahuja1, Shalini Chaturvedi2, Gullu Gorgun2, Naveen Dakappagari1, Shabnam Tangri1, Shyam Sarikonda1. 1Navigate Biopharma, Carlsbad, CA, USA.2Novartis Pharmaceuticals, Cambridge, MA, USA

4
Validation of CLL ERIC MRD Flow Cytometry panel in a CAP/CLIA-compliant model.
Marc Brugarolas1, Christele Gonneau1, Marc Lenjou2, Yoav Peretz3. 1Covance Central Laboratory Services, Geneva, Switzerland.2LabCorp BVBA, Mechelen, Belgium.3Covance Central Laboratory Services, Indianapolis, IN, USA

5
One Tube 24-colors Panel for Spectral Multicolor Flow Cytometry can Establish a Normal Myeloid Maturing and Expression Database of Bone Marrow
Man Chen1, Minjing Fu2, Aixian Wang1, Meiwei Gong1, Junyi Zhen1, Xueying Wu1, Kun Zhao2, Hui Wang1. 1Hebei Yanda Ludaopei Hospital, Langfang, China.2Beijing Ludaopei Hospital, Beijing, China

6
Pure erythroid leukemia (PEL) with aberrant lymphoid and myeloid marker expression by 10-color flow cytometry (FC) analysis and absence of TP53 protein expression: A case report
George Deeb1, Linsheng Zhang 1, Kristin Deeb1, Nikolaos Papadantonakis2. 1Emory University School of Medicine, Department of Pathology and Laboratory Medicine, Atlanta, GA, USA.2Emory University School of Medicine, Department of Hematology and Medical Oncology, Atlanta, GA, USA

7
Clinical performance equivalency between the investigational BD FACSLyric/ BD FACSDuet System and manual preparation for enumeration of T-, B-, NK lymphocyte subsets.
Yana Dutt-Singkh1, Yang Zeng1, Sixto Pacheco2, Caren Mutschmann3, Doreen Taufmann3, David Riechers3, John Saviour Carabott3, Farzad Oreizy1, Harshada Rohamare1, Angela Cheng1, Huai-Ning Chang1, Nicole DrEmpaire2, Rafael Guevara2, Mayiliex Bosch2, Maryam Saleminik1, Beverly Lu1. 1BD Biosciences, San Jose, CA, USA.2BioCollections Worldwide, Inc., Miami, FL, USA.3SYNLAB International GmbH, Berlin, Germany

8
Eosin-5'maleimide binding test of hereditary spherocytosis in Turkish population
Mesude Falay1, Seda Ayd?n2, Guchan Alanoglu3, Ebru Keskin3, Ali Fettah4, Muhittin Serdar5, Gulsum Ozet2. 1Ankara Duzen Laboratory Groups, Ankara, Turkey.2Ankara City Hospital, Ankara, Turkey.3Suleyman Demirel University Medical Faculty, Isparta, Turkey.4Health Sciences University Dr. Sami Ulus Maternity and Childrenrs Training and Research Hospital, Ankara, Turkey.5Ac?badem University Medical Faculty, Istanbul, Turkey

9
Detection of MMminimal residual disease by eight-color flow cytometry as a useful approach for analyzingimmune cell subsets and prognostic evaluationin patients withMM
Mei Han, Zhaoyun Liu, Mengran Gao, Hui Liu, Rong Fu. Department of Hematology, Tianjin Medical University General Hospital, tianjin, China

10
Single antibody evaluation of T-cell receptor beta chain monotypia by flow cytometry facilitates the diagnosis of T-cell large granular lymphocytic leukemia
Pedro Horna, Horatiu Olteanu, Dragan Jevremovic, Gregory Otteson, Heidi Corley, Wei Ding, Sameer Parikh, Mithun Shah, William Morice, Min Shi. Mayo Clinic, Rochester, MN, USA

11
IMMUNOPHENOTYPIC PROFILE OF MYELOMA PLASMA CELLS IN INTRACAVITARY FLUIDS
Maura R V Ikoma-Colturato1, 2, Felipe M Furtado2, Jessica M Bonete1, Fernanda O Resende2, Joana E Conti-Spilari1, Camila S Nobre2, Camila M Bortolucci1, Alef R Severino1, Rafael Teotonio1, Anna C M Castro2. 1Amaral Carvalho Hospital, Jau-SP, Brazil.2Sabin Medicina Diagnostica, Brasilia - DF, Brazil

12
Dynamics of aldehydedehydrogenase-positive mesenchymal number cells during cultivation
Natalia Isaeva, Konstantin Vetoshkin, Natalia Minaeva, Maria Butolina, Julia Zmeeva. Federal State-Financed Scientific Institution Kirov Research Institute of Hematology and Blood Transfusion under the Federal Medical Biological Agency, Kirov, Russia

13
The modulatory effects of a PI3kd specific small molecule inhibitor (IOA-244) on basophil degranulation measured by flow cytometry.
Eirini Kamileri1, Christele Gonneau1, Zoe Johnson2, Yoav Peretz1. 1Covance CLS, Geneva, Switzerland.2iOnctura SA, Geneva, Switzerland

14
CD34+CD133+ stem cell amount to shorten the neutrophil engraftment process for the autologous bone marrow transplantation
Mustafa Yavuz Koker, Huriye Celikzencir, Sinan Kutuk. University of Erciyes, Department of Immunology, kayseri, Turkey

15
The enumeration of endothelial stem cells (CD309+, CD146+ and CD31+) in AML blastic cells by flow cytometry
Nezihe Koker, Sinan Kutuk, Huriye Celikzencir, Mustafa Yavuz koker. University of Erciyes, Department of Immunology, kayseri, Turkey

16
Utility of MultiparametricFlow Cytometry in the diagnosis of Chronic Lymphoproliferative Disorders other than Chronic Lymphocytic Leukemia
Sindhura Lakshmi Koulmane Laxminarayana. Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Udupi, India

17
Standardized Approach for Monitoring Plasma Cell Disorders
Na Li1, Suraj Saksena2, Natalie Golts1, Chip Lomas2, Ulrika Johansson3. 1BD Biosciences, San Diego, CA, USA.2BD Biosciences, San Jose, CA, USA.3University Hospitals Bristol NHS Foundation Trust, Bristol, United Kingdom

18
CLL-1 and TIM-3 as Immunophenotypic Markers in detecting AML by Flow Cytometry
Hong Lin1, Kristin Sticco2, Ann-Leslie Zaslav1. 1Stony Brook University School of Medicine Department of Pathology, Stony Brook, NY, USA.2Northwell Health Systems Department of Hamatopathology, Greenvale, NY, USA

19
The potential diagnostic power of BCMA+/CD319+ Microparticles from the plasma analysis for multiple myeloma clinical monitoring by flow cytometry
Zhaoyun Liu, Nanhao Meng, Mengyue Tian, Rong Fu. Hematology department, Tianjin Medical University General Hospital, Tianjin, China, Tianjin, China

20
Utility of the FCM Score in Interval Marrow Studies for Patients with Myelodysplastic Syndrome
Andrew G Lytle, Jill A Johns, Julianne N Qualtieri. University of Pennsylvania, Philadelphia, PA, USA

21
The role of flow cytometry in detecting bone marrow infiltration at diffuse large B-cell lymphoma NOS diagnosis
Fernando Martin-Moro1, Claudia Lopez-Prieto2, Federico Santiago Herrera2, Isabel Delgado-Trillo2, Miguel Piris-Villaespesa1, Juan Marquet-Palomanes1, Eulalia Rodriguez1, Ernesto Roldan1, Javier Lopez-Jimenez1, Jose Antonio Garcia-Marco3, Jose Antonio Garcia-Vela2. 1Ramon y Cajal University Hospital, Madrid, Spain.2University Hospital of Getafe, Madrid, Spain.3Puerta De Hierro University Hospital, Madrid, Spain

22
Challenging flow cytometry diagnosis between gamma-delta T-cell entities in peripheral blood
Fernando Martin-Moro1, Isaac Martin-Rubio2, Isabel Delgado-Trillo2, Berta Mercedes Michael1, Federico Santiago Herrera2, Jose Antonio Garcia-Vela2. 1Ramon y Cajal University Hospital, Madrid, Spain.2University Hospital of Getafe, Madrid, Spain

23
Evaluation of associations between PAX5 gene deletions and B lymphocyte lineage antigen expression at diagnosis in pediatric B-lymphoblastic leukemia
Eric McGinnis1, Robert Guo1, Nicholas Au1, 2, Lindsay Brown1, 2, Kate M. Chipperfield1, 2, Doug Morrison1, 2, Audi F. Setiadi1, 2, Suzanne M. Vercauteren1, 2. 1University of British Columbia, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada.2BC Children's Hospital, Vancouver, BC, Canada

24
A Decision Tree Approach for Differential Diagnosis of Chronic Lymphocytic LeukemiaUsing Immunophenotyping with Flow Cytometry
Zehra Narli Ozdemir1, Mesude Falay2, Ayhan Parmaksiz2, Eylem Genc1, Ozlem Beyler1, Ahmet Kursad Gunes1, Funda Ceran1, Simten Dagdas1, Gulsum Ozet3. 1Ankara City Hospital, Ankara, Turkey.2Duzen Laboratories Group, Ankara, Turkey.3Yildirim Beyazit University School of Medicine, Ankara, Turkey

25
Characterization of Cord Blood and Leucopheresis Specimen Storage and Staining Stability Using the BD Stem Cell Enumeration Kit on the BD FACSLyric Flow Cytometer
Rakesh K Nayyar1, Anu Patel1, Sajid Dewji2, Yuanyuan Yang3, Anna Lin3, Imelda Omana-Zapata3. 1Cytoquest Corp, Toronto, ON, Canada.2University Health Network, Toronto, ON, Canada.3BD Biosciences, San Jose, CA, USA

26
Selecting the right antibody clone for flow cytometric CRLF2 detection
Roxanne Nieder, Ruth Morgan, Sa Truong, Weijie Li. Children's Mercy Hospital, Kansas City, MO, USA

27
Nonlinear Embedding Transformation of Clinical Flow Cytometry Data Reveals Atypical Cancer Cells for Computational Identification and Diagnosis of Leukemia
Yu Qian1, Quang V. Nguyen2, Preston J. Putzel3, Aishwarya Mandava1, Nicholas Bevins4, Jack Bui4, Huan-You Wang4, Padhraic Smyth3, Richard H. Scheuermann1, 4. 1J. Craig Venter Institute, La Jolla, CA, USA.2Western Sydney University, Sydney, Australia.3University of California, Irvine, CA, USA.4University of California, San Diego, CA, USA

28
Enumeration and Phenotyping of Leukemic Stem Cells in Acute Myeloid Leukemia using Spectral Flow Cytometry
Ankita Raikar1, Matthew Farren2, Todd Rogers2, Jose Estevam1, Michael Abadier1. 1Takeda Pharmaceuticals, Cambridge, MA, USA.2Q2 Solutions, Marietta, GA, USA

29
Screening B-cell Populations using a Single Bivariate Pattern Recognition Dot Plot (CD20 vs. CD38)
Amr Rajab, Afaf Erfaei , Miranda Wozniak. MedicalnScientific Department, LifeLabs Medical Laboratory Services, Toronto, ON, Canada

30
Comparing an 8-antibody liquid cocktail to an equivalent dried formulation: a quality improvement study.
Patrick J Redegeld1, Jennifer Leung1, Anne Tierens1, Clinton JV Campbell2. 1Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.2Department of Pathology, Juravinski Hospital, Hamilton, ON, Canada

31
Effects of sample-specific ten-colour panel and clinical triage elimination on flow cytometry cases turnaround time
Maci-Arielle Ricketts1, 2, Mirette Hanna1, 2, Dietrich Werner1, 2. 1Nova Scotia Health Authority, Halifax, NS, Canada.2Dalhousie University, Halifax, NS, Canada

32
A cross-standardized analytical platform to permit comparison of immunophenotypic subpopulations between acute lymphoblastic leukemia patients and their patient-derived xenografts
Nina Rolf1, Lorraine YT Liu2, Angela Tsang2, Suzanne M Vercauteren2, 3, Gregor SD Reid1, 4. 1Michael Cuccione Childhood Cancer Research Program, BC Children's Hospital Research Institute, University of British Columbia, Vancouver, BC, Canada.2Clinical Immunology Lab, Division of Hematopathology, BC Childrenrs Hospital, Vancouver, BC, Canada.3Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.4Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada

33
Diagnosing chronic granulomatous disease with dihydrorhodamine 123 flow cytometry assay
Zi Yi Shen1, Suzanne Vercauteren2, 3, Nicholas Au2, 3. 1Faculty of Medicine, UBC, Vancouver, BC, Canada.2Division of Hematopathology, BC Childrenrs Hospital, Vancouver, BC, Canada.3Department of Pathology and Laboratory Medicine, UBC, Vancouver, BC, Canada

34
Validation of BD FACSDuetTM Flow Cytometry Sample Preparation System
Joanna Sin, John Soltys. St Michaels Hospital, Unity Health Toronto, TORONTO, ON, Canada

35
Challenges in validating of inducible basophil biomarkers: Time, Temperature and Transportation
Soren Ulrik Sonder1, Theodore Kim2, Jing Yu3, Matthew Plassmeyer1, Oral Alpan1. 1Amerimmune LLC, Fairfax, VA, USA.2Allergy Partners, Chantilly, VA, USA.3Institute for Asthma and Allergy, Wheaton, MD, USA

36
Analysis of effect of honey and cigarette smoke on lung inflammation by using flow cytometry
Minoru Takeuchi1, Honami Nakata1, Kent Pinkerton2. 1Kyoto Sangyo University, Kyoto, Japan.2UCD, Davis, CA, USA

37
Malignant PCs in bone marrow detected by flow cytometry as a predictor for the risk stratification system of multiple myeloma
Mengyue Tian, Zhaoyun Liu, Hui Liu, Rong Fu. Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China

38
A New Method to Detect 24 Cytokines in Peripheral Blood Post Chimeric Antigen Receptor-T Cell Therapy by Flow Cytometry and Microbeads
Dongchu Wang, Xiang Li, Liyuan Qiu, Shizhe Fu, Lingyun Cui, Hui Wang. Hebei Yanda Ludaopei Hospital, Langfang, China

39
A Rare Case of Acute Monocytic Leukaemia Co-occurred with Relapsed Neuroblastoma
Hui Wang, Aixian Wang, Ping Wu, Junyi Zhen, Xueying Wu, Meiwei Gong, Tong Wang, Hongxing Liu. Hebei Yanda Ludaopei Hospital, Langfang, China

40
Evaluation of Sysmex Kappa and Lambda Monoclonal Antibodies for the Determination of B-Cell Surface Light Chains
Gavin Williams1, Rosalie Ward1, Sheriaz Bashir1, Alison Whitby2, Matthew Fletcher2, Richard Wardle1. 1Sheffield Teaching Hospitals Foundation Trust, Sheffield, United Kingdom.2UK NEQAS for Leucocyte Immunphenpotyping, Sheffield, United Kingdom

41
Ogata Scores Show Similar Performance Across Platforms in Predicting Myelodysplastic Syndromes
Margaret C Williams1, 2, Peng Li1, 2, David P Ng1, 2. 1ARUP Laboratories, Salt Lake City, UT, USA.2University of Utah, Salt Lake City, UT, USA

42
A case report of residual T cell acute lymphoblastic leukemia detected by back-gate analysis of a novel 10 colour/15 antibody flow cytometry screen tube
Richard Xiang1, 2, Ian Grace1, Mahboubeh Rahmani1, 2, Dietrich Werner1, 2. 1Nova Scotia Health Authority, Halifax, NS, Canada.2bDalhousie University, Halifax, NS, Canada

43
Evaluation of the Long-Term Stability of the BD CS&T Setup Method on the BD FACSLyric System
Yang Zeng, Angela Chen, Harshada Rohamare, Farzad Oreizy. BD Biosciences, San Jose, CA, USA

44
Case Report: Flow Cytometry Immunophenotype Mimicking Plasmacytoid Leukemia/Lymphoma in an AITL
Zhenghong Zhu, Yaping Wu. Providence Regional Leb, Portland, OR, USA