ICCS 2024 | Seattle, WA

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Conference Program

Conference Program

The Course will only be offered in-person.
The Meeting will be available both in-person and virtually.

All program times are listed in Pacific Time zone.

COURSE DATES
Friday, October 4 - Sunday, October 6
The course is scheduled to finish at noon (12pm) on Sunday, October 6.

MEETING DATES
Sunday, October 6 - Tuesday, October 8
The meeting will offically start Sunday, after the corporate workshops, at 2pm.

Fri - Oct 02

Sat - Oct 03

Sun - Oct 04

Mon - Oct 05

Tue - Oct 06

View Program Details

Monday, October 5th

8:00 - 8:30 AM
Breakfast

BREAKFAST

Symphony 2-4

8:15 - 8:30 AM
Meeting

BUSINESS MEETING - OPEN TO ALL ICCS MEMBERS

Symphony 1

8:30 - 11:30 AM
Plenary

PLENARY 2 - DIAGNOSTIC FLOW
Chair/Organizer: Cara Shirai, Fabienne Lucas

Symphony 1

8:30

CSI Case
Vorapat Vorapanya.

8:45

The Evolving Role of Flow Cytometry in Bone Marrow Evaluation: Insights from the Bone Marrow Pathology Working Group
Olga Weinberg.

9:15

Recent developments in NK cell immunophenotyping, including NK cell receptor restriction, KIRs and other approaches in diagnostic flow cytometry
Aaron Wilk.

9:50

Exhibits & Coffee Break
.

10:20

Diagnostic advances and CD38-centered strategies in multiple myeloma treatment and monitoring in the era of targeted therapies
Afshin Shameli.

10:55

Small Cells, Big Signals: Flow Cytometry for Platelet Disorders
Benjamin Spurgeon.

11:30 - 12:30 PM
Luncheon Workshop

LUNCH WORKSHOP 1: PUBLISHING YOUR FLOW CYTOMETRY WORK (IN-PERSON ONLY)

Acapella 1

11:30

Publishing your flow cytometry work - Part 1
Joe DiGiuseppe.

12:00

Publishing your flow cytometry work - Part 2
Fred Preffer.

LUNCH WORKSHOP 2: T-CELL LYMPHOMA DIAGNOSTICS INCLUDING TRBC1 AND TRBC2 (IN-PERSON ONLY)

Concerto C

11:30

T-cell lymphoma diagnostics including TRBC1 and TRBC2
Chrysanthi Tsamadou.

LUNCH WORKSHOP 3: HIGH SENSITIVITY FLOW CYTOMETRY FOR DIAGNOSIS AND MONITORING OF PEDIATRIC MYELOID NEOPLASMS AND BONE MARROW FAILURE SYNDROMES (IN-PERSON ONLY)

Concerto B

11:30

Inherited Bone Marrow Failure Syndromes & Flow Cytometry Clues
Lisa Brodersen.

LUNCH WORKSHOP 4: SPECTRAL FLOW CYTOMETRY- PRACTICAL APPLICATIONS (IN-PERSON ONLY)

Concerto A

11:30

Practical Considerations for Implementing Clinical Spectral Flow Cytometry- Part 1
Joe Lownik.

12:00

Practical Considerations for Implementing Clinical Spectral Flow Cytometry- Part 2
Jean Oak.

1:00 - 4:00 PM
Plenary

PLENARY 3 - FLOW LAB TECHNOLOGY (JOINT ICCS/ESCCA)
Chair/Organizer: Leo Lin, Jolene Cardinali

Symphony 1

1:00

CSI Case: Unmasked Systemic Mastocytosis in AML with Multilineage Myeloid Differentiation
Mariam Posso, David Czuchlewski, Evelyn Lockhart, Daniel Babu.

1:20

Harmonizing Spectral Flow Cytometry in the Clinical Lab: A Call for a Standing Working Group - Part 1
Jean Oak.

1:45

Harmonizing Spectral Flow Cytometry in the Clinical Lab: A Call for a Standing Working Group- Part 2
Joe Lownik.

2:10

Exhibits & Coffee Break
.

2:50

ARUP Laboratory's Experience with Rarity's SuperRCA®Molecular Flow Assay
Leo Lin.

3:25

Functional precision medicine predicts outcome and therapy response in AML
Jeremy Ryan.

4:00 - 5:30 PM
Posters/Exhibits

POSTER SESSION 2

Symphony 2-4

200

Concurrent PTCL-NOS, EBV-Positive B-Cell Lymphoma, and CAR-T Cells After Cellular Therapy
Lily Benedict, Shazia Khan, Katherine Devitt. University of Vermont Medical Center, Burlington, VT, USA

201

Bilinial Mixed Phenotype Acute Leukemia of Myeloid and Lymphoid origin
Eric Carter, Kevin Stephen, Brianna Waller. Mass General Brigham, Boston, MA, USA

202

CD3 Detection After Bispecific Antibody Therapy: Odronextamab Interference with Lymphocyte Subset Analysis
Tori Catey, Sindhu Cherian, Valerie Miller, Fabienne Lucas. University of Washington, Seattle, WA, USA

203

Analytical Performance of the DxFLEX 13-Color Flow Cytometer for Measurable Residual Disease in B-Lymphoblastic Leukemias/Lymphomas
Raidelys Avila Diaz¹, Shrey Sharma¹, Arpan Samaddar¹, Yun Chong Sawyer¹, Nisha Patel¹, Guang Fan¹, Wei Xie¹. ¹Oregon Health & Science University, Portland, OR, USA.², .³, .⁴, .⁵, .⁶, OR.⁷,

204

Comparative analysis of flow immunophenotypes between benign thymus hyperplasia and cortical T-LBL
Qing Du, Aixian Wang, Hui Wang. Hebei Yanda Lu Daopei Hospital, Langfang, China

205

Bringing Molecular MRD to the Hematology Lab: Direct-from-Blood superRCA Assay on Standard Flow Cytometers
Tomas Edgren, Lei Chen. Rarity Bioscience, Uppsala, Sweden

206

Plasmablastic Lymphoma in the Immunocompetent Male: Two Cases with Flow Cytometric Findings
Sarah E. Hay¹, Isaac McCool^{1, 2}, Mukai Nobuyuki³. ¹San Antonio Uniformed Services Health Education Consortium, San Antonio, TX, USA.²Walter Reed National Military Medical Center, Bethesda, MD, USA.³National Defense Medical College, Tokorozawa, Japan

207

A Novel Whole Blood Stabilization Tube for the Preservation of Resting and Adenosine Diphosphate Activated Platelet P Selectin Expression
Yuqian Hua¹, Kelvin Liu², Xingzhou Liu², Zhaohui Wang². ¹IntelliFlow (Beijing) Biotechnology Co., Ltd., Beijing, China.²Gene tech ents Ltd., Princeton, NJ, USA

208

Long-Term Whole Blood Stabilization Preserves Immune Phenotypes for Multiparameter Cytometry
Carly/L Lancaster, Kristina Magee, Landon Wellendorf, Sacha Martiny, Hannah Selken, Justin Jarrell, Ramji Srinivasan, Li-Chun Cheng. Teiko, Salt Lake City, UT, USA

209

Automated Antibody Cocktailing Reduces Operator- and Site-Dependent Variability in High-Parameter Flow Cytometry Panel Preparation
Melvin Lye¹, Sashigala Ponnalagu¹, Amira A. Amiruddin¹, Kavita Mathi², Holden Maecker², Chyan Ying Ke¹. ¹Curiox Biosystems, Woburn, MA, USA.²Human Immune Monitoring Center, Stanford University School of Medicine, Stanford, CA, USA

210

Validation of Customized Synthetic Cell Mimics for Standardized Quality Control of Multidimensional Flow Cytometry Assays
Mohau S. Makatsa, Jenna M. Perry, Minh Phan, Thanh Thanh T. Dinh, Andrew J. Menssen. Hematologics, Seattle, WA, USA

211

Artificial Thymic Organ Culture Complementary Diagnostic for T-cell Lymphopenia and Congenital Athymia
Sharon Manley¹, Elie Haddad², Luigi Notarangelo³, Clara Soulard², Khampoun Sayasith ², Marita Bosticardo³, Francesca Pala³, Emily Brooks⁴, Jennifer Woo⁴, Sarah Nicholas⁴, Steve Warner⁴, Laura Canaday¹, Thomas Beadnell¹, Virginia Litwin¹. ¹Eurofins Viracor BioPharma Services, Lenexa, KS, USA.²CHU Sainte-Justine Azrieli Research Center, University of Montreal, Montreal, QC, Canada.³National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.⁴Sumitomo Pharma America, Inc., Cambridge, MA, USA

212

Evaluation of a Standardized Flow Cytometry Workflow for Basophil Activation Testing in Allergy Research
Swetha Mohan, David PILLON, Anagha Divekar. BioLegend Inc, San Diego, CA, USA

213

superRCA - Enabling ultra-sensitive mutation detection on flow cytometry
Amey Mukim, Tomas Edgren, sofia Nordin, Irene Cancela, Marie Engvall, Lei Chen. Rarity Bioscience, Uppsala, Sweden

215

Advancing T-Cell clonality assessment in clinical flow cytometry with Anti-TRBC1 and Anti-TRBC2 Antibody Conjugates
K Ralhan¹, J Blanc², X Pineau², S Bourdet², C Zambeaux², G BrÃ©tÃ©chÃ©², J Vernier², F Monsonis², N Dupas², M Burgaud², C Bodi², B Ezzouaouy². ¹Beckman Coulter Life Sciences, Bangalore, India.²Beckman Coulter Life Sciences, France, France

216

Hemodilution is the Primary Cause of Discordance Between Morphologic and Flow Cytometric Myeloid Blast Estimates in Bone Marrow Aspirate Samples.
Ntasha Roy¹, Jerry Radich², Min Fang², Xiaoyu Qu², Cecilia Yeung², Shahanawaz Jiwani³, Megan Othus², Harry Erba⁴, Mark Litzow⁵, Lyndsay Harris³, Richard Little³, Brent Wood¹. ¹Childrenâs Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.²Fred Hutchinson Cancer Research Center, Seattle, WA, USA.³National Cancer Institute/National Institute of Health, Bethesda, MD, USA.⁴Duke University, Durham, NC, USA.⁵Mayo Clinic, Rochester, MN, USA

217

Interpretive Challenges of PNH Flow Cytometry in GATA2 Deficiency: GPI-Deficient Populations in Two Patients
Fatima S. Sattar, Eduard Matkovic, Jen L Salvo, Jenny Byrd. Department of Pathology and Laboratory Science, Madison, WI, USA

218

Pre-Analytic Stabilization of Bone Marrow Aspirates for Acute Myeloid Leukemia Measurable Residual Disease Testing by Spectral Flow Cytometry
Lily Shang¹, Aaron Logan², Emma Searle³, Karen Rees-Unwin⁴, Thanuja Gali Ramamoorthy⁴, Olaf Zoellner⁵, Carly Lancaster¹, Sacha Martiny¹, Arya Iyer¹, Sergey Ingram¹, Landon Wellendorf¹, Justin Jarrell¹, Ramji Srinivasan¹, Li-Chun Cheng¹. ¹Teiko, Salt Lake City, UT, USA.²UCSF, San Francisco, CA, USA.³The Christie NHS Foundation Trust, Manchester, United Kingdom.⁴University of Manchester, Manchester, United Kingdom.⁵Cytek Biosciences, Fremont, CA, USA

219

From Four Tubes To One: A Double-Blinded Evaluation Of A 20-Color Spectral Acute Myeloid Leukemia Panel
Paul K. Wallace^{1, 2}, Orla Maguire¹, Joseph D. Tario, Jr.¹, You-Wen Qian¹. ¹Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.²SciGro, Inc., Sedona, AZ, USA

220

Validation of ISHAGE-based CD34 stem cell enumeration on the Agilent NovoCyte Penteon using direct volumetric counting
Daniel Woods¹, Lyndsey Feehan¹, Jeri Dorsey¹, Leslie Koutoufaris¹, Priyanka Pandey¹, Michele Paessler^{1, 2}. ¹Clinical Immunology Lab, Abramson Research Center, Childrenâs Hospital of Philadelphia, Philadelphia, PA, USA.²Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

221

Previously healthy 63-year-old woman presenting with fever, pneumonia, hepatosplenomegaly, and pancytopenia
Vanessa J Dayton¹, Dang H Thien², Sang Nguyen³. ¹Impact Health Vietnam, Bloomingon, MN, USA.²Ho Chi Minh City BTHH, Ho Chi Minh City, Vietnam.³Ho Chi Minh City BTHH, Ho Chi Minh City, Vietnam

222

A case of Blastic Plasmacytoid Dendritic Cell Neoplasm, a rare mimicker of acute myeloid leukemia.
Joy Hoang, Taylor Zak. Loyola University Medical Center, Maywood, IL, USA

223

MT2A::MLLT3-Rearranged B-Lymphoblastic Lymphoma Mimicking High-Grade B-Cell Lymphoma in an Infant
Crystal Xutong Liu. NYU Langone Medical Center, New York, NY, USA

224

High-Grade B-Cell Lymphoma with BCL2 and MYC Gene Rearrangements “Double-Hit” Mimicking Acute Leukemia, with CD117 Expression
Vandana Panakkal², Bryan Rea^{1, 2}, Sara Monaghan^{1, 2}, Ahmad Al-Attar^{1, 2}, Nana Matsumoto^{1, 2}, Majd Jawad^{1, 2}. ¹University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.²University of Pittsburgh Medical Center, Pittsburgh, PA, USA

225

Flow Immunophenotypic Features of Maturing Thymocytes Within a Type AB Thymoma: A Case Report and Literature Review.
Max N. Rogers, Isaac E. McCool, Robert J. Kitz. Department of Pathology, Walter Reed National Military Medical Center, Bethesda, MD, USA

226

Phenotypic Plasticity in Acute Myeloid Leukemia During Venetoclax and Azacitidine Therapy
Shrey Sharma , Wei Xie. Oregon Health & Science University, Portland, OR, USA

227

Flow Cytometric Characterization of T-Cell Prolymphocytic Leukemia with TCL1A Rearrangement and JAK1/JAK3 Mutations in an Elderly Patient
Carlos J Villarroel^1.2, Dmitry Zlobin^1.2, Deborah A Fuchs^1.2. ¹University of Arizona - Department of Pathology and Laboratory Medicine, Tucson, AZ, USA.²Banner University Medical Center Tucson, Tucson, AZ, USA

228

FlowSimon: A Broadly Applicable Clinical Decision Support System for Automated Leukemia/Lymphoma Flow Cytometry Analysis
Kamraan Gill^{1, 2}, Elizabeth Kagotho⁶, Feras Ally⁴, Ashley Volaric⁵, Steven Kussick³. ¹Zynasat, Fairfield, CT, USA.²AmeriPath, Shelton, CT, USA.³PhenoPath Laboratories, Seattle, WA, USA.⁴HemaPath Consulting, Reno, NV, USA.⁵Department of Pathology and Laboratory Medicine, University of Vermont Larner College of Medicine, Burlington, VT, USA.⁶Aga Khan University Hospital, Nairobi, Kenya

229

Double-Negative T-Cell Large Granular Lymphocytic Leukemia with a FAS Mutation: A Novel Autoimmune Lymphoproliferative Syndrome–Like Pathobiologic Presentation
Sameer Andani, Jamie E Rogers, Sara Niyazi, Mingfei Yan, Christopher B Ryder, Bijal D Shah, Dietrich WernerIdiaquez, Sara M AbuMehsen. Moffitt Cancer Center University of South Florida, Tampa, FL, USA

230

Nucleated Cells Versus Forward Scatter/Side Scatter–Derived Denominator for Flow Cytometric Measurable Residual Disease Assessment in B-Lineage Acute Lymphoblastic Leukemia: Strong Correlation Does Not Imply Interchangeability.
KARTHIK BOMMANNAN BOMMANNAN KUPPURAJU, Shirley Sundersingh. Cancer Institute (W.I.A), Chennai, India

231

Relapsed MDS Post Allogenic Stem Cell Transplant Identified by Peripheral Blood Flow cytometry
Samuel V Buskirk, Kyle Tripp, Rajeswari Jayakumar. Virginia Commonwealth University, Richmond, VA, USA

233

Cytoplasmic TRBC1 and 2 are Useful for Evaluating T-LL MRD and Mature T Cell Lymphoma
Valerie Woodings, Orrin Koepke, Shin La Shu, Afshin Shameli, Fabienne Lucas, Sindhu Cherian, Jonathan Fromm. Department of Laboratory Medicine and Pathology, University of Washinton, Seattle, WA

234

Utility of a 12-color flow cytometry assay to evaluate T cell clonality and immunophenotype T follicular helper populations
Valerie Woodings, Orrin Koepke, Paul Simonson, Afshin Shameli, Fabienne Lucas, Sindhu Cherian, Jonathan Fromm. Department of Laboratory Medicine and Pathology, University of Washinton, Seattle, WA

235

Ultra-Sensitive Multiplex Detection of KIT D816 Variants Using Multi-color superRCA Assay
Irene GolÃ¡n Cancela¹, Mark Rosenzweig², Rentian Wu², Guang Yang², Lei ChenÂ¹¹. ¹Rarity Bioscience AB, Uppsala, Sweden.²Blueprint Medicines, Boston, MA, USA

236

FlowAID: An Auditable, LLM-Powered application for Automated Flow Cytometry Specimen Triage
Sulov Chalise, Shu Jie Zhang, Qi Gao, Matthew Thomsen, Lulu Choe, Jennifer Reminick, Anyi Li, Mikhail Roshal, Menglei Zhu. Memorial Sloan Kettering Cancer Center, New York, NY, USA

237

Synthetic CD34 Stem Cell Mimics as a Stable and Reproducible Control for Clinical Flow Cytometry Applications
Swetha Pratyusha Gunturu, Subhanip Biswas, Valerie Chen, Kanwal Palla. Slingshot Biosciences, Inc., Emeryville, CA, USA

238

Reproducible 12-Color Immunophenotyping of Human Whole Blood Using SpectraComp™ Unmixing and Compensation Particles on the BD FACSLyric™
Lorena Hidalgo¹, Ai-Li Wei¹, Shilpi Verma¹, Rodrigo Pestana², Stephanie Widmann¹. ¹Waters Biosciences, San Diego, CA, USA.²Waters Biosciences, Milpitas, CA, USA

239

From Discovery to Analytical Confidence
Tri Le, Chip Lomas, Abigail Kelliher, Aaron Middlebrook. Waters Biosciences, Milpitas, CA, USA

240

Reproducibility on Research Spectral Cytometers: Cross-Platform Evaluation Against the FACSLyric™ Gold Standard
Tri Le, Chip Lomas, Aaron Middlebrook. Waters Biosciences, Milpitas, CA, USA

241

A Novel Anti-TCRα/β Antibody for Enhanced TCR β Clonality Evaluation
Derrick Lee, Takatoku Oida, Michael Li. BioLegend Inc, San Diego, CA, USA

242

An Auditable Machine-Learning Workflow with Specimen-Adaptive Cleanup for Lymphocyte Subset Analysis
Shreya Nagesh, Uyen Pham, Sumire Kitahara, Joseph Lownik. Cedars-Sinai Medical Center, Los Angeles, CA, USA

243

Beyond the frozen algorithm: a tripartite human–algorithm–LLM framework for flow-cytometric MRD in AML
GEORGIOS PATERAKIS¹, GEORGIOS OUDATZIS¹, ANGELIKI KOTSIAFTI¹, MAROULA MILAIOU¹, KATERINA MANDARAKA¹, PARASKEVI VASILEIOU². ¹IMMUNOLOGY DEPARTMENT, "GEORGIOS GENNHMATAS" GENERAL HOSPITAL, ATHENS, Greece.²FLOWDIAGNOSIS DIAGNOSTIC LABORATORY, ATHENS, Greece

244

From Conventional to Spectral Flow Cytometry: Practical Lessons from Clinical Laboratory Implementation
Uyen Pham, Leslie Layco, Melissa Cervania, Allan Gayacao, Joseph Lownik M.D., Sumire Kitahara M.D.. Cedars Sinai Medical Center, Los Angeles, CA, USA

245

Clinical Validation of a Single-Tube 39-Parameter Spectral Flow Cytometry Assay for Pediatric T-ALL MRD
Stacie Woolard¹, Laura Key¹, Peixin Liu¹, Christy Embrey¹, Zhaohong Yin¹, Yen-Chun liu¹, Mahsa Khanlari¹, Jeffery/M Klco¹, Priyadarshini Kumar², Paul/E Mead¹. ¹St. Jude Children's Research Hospital, Memphis, TN, USA.²Memorial Sloan Kettering Cancer Center, New York, NY, USA

246

Stability and Reproducibility of a Commercial Cellular Control Versus Whole Blood for Flow Cytometric Immunophenotyping
Hannah Micek, Stephanie Wigginton. Streck, La Vista, NE, USA

5:30 - 6:15 PM
Meeting

MEET THE COMMITTEES (OPEN TO ALL ICCS MEMBERS)

Concerto A

7:00 - 9:00 PM
Social Event

SOCIAL NETWORKING EVENING

Soundgarden