ICCS eNewsletter


International Clinical Cytometry Society		VOL. II No. 4, Fall 2011


Case Study Interpretation (CSI)
Clinical History: The patient is a 70-year old female presented with mild leukocytosis. She has no prior history of any significant diseases and is asymptomatic. Bone marrow (BM) aspirate and biopsy were procured. Flow cytometric analysis was performed on marrow aspirate and results from selected 4-color tubes are provided for review. Acquisition: Beckman Coulter Epics XL (FCS2.0, System II). Analysis: Paint-A-Gate software (adapted to Coulter). Tubes (FITC/PE/ECD/PC5): Tube 1: Kappa/lambda/45/19+20 Tube 2: 5/19/45/10 Tube 3 : 8/4/45/38 Tube 4: 15/117/45/34 Tube 5: 20/10/19/38 Tube 6: FMC-7/23/5/19 Tube 7: Kappa/Lambda/5/19 Download list mode files Download example analysis (PDF format) Case discussion (PPT) Weina Chen, MD, PhD Ameripath/Quest Diagnostics Dallas, Texas

In This Issue

Editorial

ICCS Portland Plenary Session I: Regulatory Issues

ICCS Portland Plenary Session II: Optimization of Multicolor Immunophenotyping Assays: Critical Components and Practical Guidelines

ICCS Portland Plenary Session III: International Focus

ICCS Portland Plenary Session IV: Hematopathology

ICCS Portland Plenary Session V: Translational Medicine

Case Study Interpretation (CSI)

Archive

Would You Like to Contribute a Case Study?

You will need to provide:

1. A brief case history including demographic data, specimen type, relevant clinical history, and indication for study.

2. A list of the tubes selected for review with the antibodies and fluorochromes used.

3. Selected anonomized list mode files to post on the ICCS website. List mode files should be labeled with a key detailing antibodies and fluorochromes used, the format in which files were collected (FCS2.0 or FCS3.0, eg) and the software used for collection.

4. PDF files for each tube selected, showing an example of what a correct and complete analysis should look like.

5. A Powerpoint presentation summarizing your case, to include a description of a recommended analysis strategy, the flow cytometric pertinent findings, and their significance.

6. A passport-style photo of each author.

7. A signed web consent form.