International Clinical Cytometry Society

Case Study Interpretation (CSI)


Clinical History:

An 8 year old female presenting with fever and bruising on extremities.

CBC and differential in tables

CBC parameter Result Units Reference Range
WBC 122.2 x109/L (5.41 - 9.70)
RBC 3.01 x1012/L (3.88 - 4.72)
HGB 8.4 g/dl (11.3 - 13.4)
HCT 25.3 % (32.3 - 38.3)
MCV 84.0 fl (79.5 - 85.2)
MCH 27.9 pg (27.8 - 30.0)
MCHC 33.4 gm/dl (34.3- 35.8)
RDW 13.9 % (12.8 - 13.9)
PLT 86 x109/L (187 - 376)

   
WBC Differential % Absolute Units Reference Range (Absolute)
Neutrophils 0 0 x109/L (2.58 - 5.95)
Lymphocytes 8


Monocytes 1


Eosinophils 0


Others 91


  
A bone marrow aspirate and biopsy were procured.

Flow cytometric analysis was performed on the marrow aspirate and results from selected 4-color tubes are provided for review.

Flow Cytometric Files:                                                

Acquisition FACS CantoII, analysis DIVA (FITC / PE / PerCP-Cy5.5 / APC)
Tube 1: HLA-DR / CD117 / CD45 / CD19
Tube 2: CD13+CD33 / CD7 / CD19 / CD2
Tube 3: CD20 / CD10 / CD45 / CD19
Tube 4: Lambda / Kappa / CD19 / CD22
Tube 5: CD15 / CD56 / CD19 / CD34

The listmode files are available in 2 ways FCS 2.0 files and FCS 3.0 files.
Download FCS 2.0 files
Download FCS 3.0 files

Download example analysis (PDF files):
CSI Winter 2011-BMA_001
CSI Winter 2011-BMA_002
CSI Winter 2011-BMA_003
CSI Winter 2011-BMA_004
CSI Winter 2011-BMA_005

Download case discussion (PDF file).


Jo-Anne Vergilio, MD
Director, Hematopathology
Medical Director, Flow Cytometry (Oncology Division)
Children's Hospital Boston
Department of Pathology
Boston, MA



Would You Like to Contribute a Case Study?


You will need to provide:

1. A brief case history including demographic data, specimen type, relevant clinical history, and indication for study.

2. A list of the tubes selected for review with the antibodies and fluorochromes used.

3. Selected anonomized list mode files to post on the ICCS website.  List mode files should be labeled with a key detailing antibodies and fluorochromes used, the format in which files were collected (FCS2.0 or FCS3.0, eg) and the software used for collection.

4. PDF files for each tube selected, showing an example of what a correct and complete analysis should look like.

5. A Powerpoint presentation summarizing your case, to include a description of a recommended analysis strategy, the flow cytometric pertinent findings, and their significance. 

6. A passport-style photo of each author.

7. A signed web consent form.