ICCS 2025 | Philadelphia, PA

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Course & Meeting Program

Course & Meeting Program

Please find the 2025 Meeting & Course Program below.

The course will take place on Friday and finish at noon (12pm) on Sunday.
The meeting will then start on Sunday afternoon and finish on Tuesday.

All program times are listed in the Eastern Time zone.

View Summary Only

Sunday, September 28th

7:30 - 8:00 AM
Break

BREAKFAST

Commonwealth Foyer

7:30 - 10:30 AM
Break

COURSE REGISTRATION

Foyer

8:00 - 11:50 AM
Course

COURSE SUNDAY AM

Commonwealth BCD

8:00

Guidance and Suggestions for Managing a Flow Lab - Part 1
Andrea Illingworth.

8:20

Guidance and Suggestions for Managing a Flow Lab - Part 2
Bruce Greig.

8:45

T/NK Cell Neoplasms
Min Shi.

9:45

Coffee Break
.

10:00

Plasma Cell Neoplasms
Mike Linden.

10:45

Future of Flow - AI
Joseph DiGiuseppe.

11:20

Course Speaker Forum
.

11:40

Course Wrap-Up
Mike Linden.

11:30 - 8:00 PM
Break

MEETING REGISTRATION

Foyer

12:30 - 2:30 PM
Corporate Workshop

CORPORATE WORKSHOPS
Chair/Organizer: Adam Seegmiller

Commonwealth A1

12:30

Cytek Bio
.

1:00

BD
.

1:30

Beckman Coulter
.

2:00

Sysmex
.

2:30 - 5:10 PM
Plenary

MEETING WELCOME & PLENARY 1 - REGULATORY AND TECHNICAL CHALLENGES IN CLINICAL FLOW CYTOMETRY
Chair/Organizer: Adam Seegmiller, Wolfgang Kern

Commonwealth BCD

2:30

Meeting Welcome
Sa Wang.

2:45

CSI Case
Nourhan Ibrahim.

3:00

Daily Quality Control of Flow Cytometry Panels - Part 1
Robert Seifert.

3:10

Daily Quality Control of Flow Cytometry Panels - Part 2
Giovanni Insuasti.

3:20

LDTs - Lessons Learned and Future Directions
Ajay Grover.

3:45

Coffee Break
.

4:20

Flow Cytometry of Paucicellular Specimens
Marie Christine Bene.

4:45

Implementation of Flow Cytometry in Rare Matrix Samples
Madhu Ouseph.

5:15 - 6:00 PM
Keynote

WALLACE H. COULTER LECTURE
Chair/Organizer: Adam Seegmiller

Commonwealth BCD

5:15

Wallace H Coulter Introduction
Adam Seegmiller.

5:20

Reflections, Relationships, and Lessons Learned
Bruce Greig.

6:00 - 7:30 PM
Posters/Exhibits

POSTER SESSION 1 & EXHIBIT RECEPTION (click to view) (click to close)

Millennium Hall

101

Performance Evaluation of the DxFLEX Flow Cytometer for Crossmatch Testing in Histocompatibility
Laiz C. Bento¹, Jobson F. Nascimento², Flavia A. Sousa¹, Margareth M. Torres^{1, 2}, Elizabeth X. Souto¹, Nydia S. Bacal¹. ¹Hospital Israelita Albert Einstein â Flow Cytometry â Histocompatibility, SÃ£o Paulo, Brazil.²Hospital Israelita Albert Einstein â Histocompatibility, Recife, Brazil

102

Genetic landscape of Acute Myeloid Leukemia with downregulation of CD38 median fluorescent intensity in Leukemic blasts
Shreyam Acharya, Richa Chauhan, Jasmita Dass, Preeti Khokhar, Ganesh KV, Mukul Aggarwal, Pradeep Kumar, Rishi Dhawan, Tulika Seth, Seema Tyagi, Manoranjan Mahapatra. All India Institute of Medical Sciences, New Delhi, India

103

Detection of Rare and Low-Expressing BCMA CAR-T Cells Using a 1-Step Flow Cytometry Reagent
Joseph Cantor, Arthi Kannan, Gary Yao, Omar Bazirgan, Wei Fan, Megan Pawarna, Gisele Baracho, Christian Carson, Shilpi Verma. BD Biosciences, San diego, CA, USA

104

FLOW CYTOMETRIC ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES AFTER CAR-T-CELL THERAPY
Kate Claypool, Andrew Conoley, Katalin Kelemen, Jiehao Zhou. Mayo Clinic Hospital, Phoenix, AZ, USA

105

Comparison of Phosphatidylserine-Positive Live Sperm Between Pregnancy-Proven Individuals and Individuals Seeking Fertility Treatment
Leah Dobson, Bindica Poudel, Christina George, Thomas Alexander, Jeffrey Lysiak. PS Fertility, Charlottesville, VA, USA

106

A rapid QC method to assess antibody cocktail for use in natural killer cell degranulation (CD107a) assay in a clinical immunology lab
Lyndsey Feehan¹, Leslie Koutoufaris¹, Jeri Dorsey¹, Michele Paessler^{1, 2}, Priyanka Pandey¹. ¹Clinical Immunology Lab, Abramson Research Center, Childrenâs Hospital of Philadelphia, Philadelphia, PA, USA.²Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

107

Optimizing Harmonized Flow Cytometry Panels: The Role and Importance of Backbone Markers in Multi-Panel Experiments for Clinical Trials.
Ekambaram Ganapathy¹, Taryn Mockus¹, Xiao Li¹, Thea Hogan², Irene del Molino del Barrio², Suchete Hunjan², Esther Perez Garcia², Max Kirtlan², Ruth Barnard², Vilma Decman^{1, 2}, Veronica Nash¹. ¹Cellular Biomarkers Group, BBP, GSK, Collegeville, PA, USA.²Cellular Biomarkers Group, BBP, GSK, Stevenage, Hertfordshire, SG1 2NY, United Kingdom

108

Comparative Evaluation of Whole Blood Preservation Methods for High-Parameter Flow Cytometry in Clinical Trials
Ciara Gimblet-Ochieng¹, Xiao Li¹, Krishnendu Chakraborty², Taryn Mockus¹, Ruth Barnard², Vilma Decman¹, Veronica Nash¹. ¹GSK, Collegeville, PA, USA.²GSK, Stevenage, United Kingdom

109

Advancing GPRC5D Detection in Multiple Myeloma: A Novel GPRC5D-targeted Antibody Developed to Enable Precise Detection in Clinical Research
Ru Huang, Hongfei Wang, Miaoling Zhang, Haiyou Gao, Mira Turgun, Liang Da. Cytoart, Inc, Tucson, AZ, USA

110

Successful validation of a 30-color flow cytometer for clinical application
Leslie Koutoufaris¹, Joseph McMann¹, Brandon Goff¹, Melvin Barclay¹, Rosalie Stetz¹, Priyanka Pandey¹, Michele Paessler^{1, 2}. ¹Clinical Immunology Lab, Abramson Research Center, Childrenâs Hospital of Philadelphia, Philadelphia, PA, USA.²Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

111

Comparison of Whole Blood Preservation Methods for Cytometric Analysis
Carly Lancaster, McKenna Coughlin, Gaga Black, Justin Jarrell, Ramji Srinivasan, Li-Chun Cheng. Teiko Bio, Salt Lake City, UT, USA

112

Immunophenotypic and Molecular Characterization of BRAF-Mutated Myeloid Neoplasms
Elisa Lin, Kenneth Chen, Alexa AndrÃ©, Sindhu Cherian, Alireza Torabi. University of Washington, Seattle, WA, USA

114

Index Gating: Automated Boolean Landmarks for Streamlining CD45/SSC Gating
Christopher G. Snyder^{1, 2}, Cara L. Shirai¹. ¹Washington University in St. Louis, St. Louis, MO, USA.²University of Washington, Seattle, WA, USA

115

Single-Tube Approach for Robust Compensation Matrix Generation in High-Dimensional Flow Cytometry: Eliminating the Need for Individual Single-Color Controls
Xiaotian Sun, Sary EI daker, Qi Gao, Mikhail Roshal. Memorial Sloan-Kettering Cancer Center, Department of Pathology and Laboratory Medicine, Hematopathology Service, New York, NY, USA

116

DIAGNOSIS OF EBV INFECTION ON LYMPHOCYTE SUBSETS IN HEMOPHAGOCYTIC LYMPHOHISTIOCYTOSIS AND BENIGN EBV-RELATED HEMATOLOGICAL DISORDERS: FLOW CYTOMETRY OR REAL-TIME PCR
Mai Anh Thi Nguyen¹, Truong Nhat Trinh^{1, 2}, Tuan Gia Doan Nguyen^{1, 2}, Minh Tuyet Hoang Nguyen¹. ¹University of Medicine and Pharmacy at HCMC, Ho Chi Minh, Vietnam.²MICOL Institute, Ho Chi Minh, Vietnam

117

Prognostic value of T-lymphocyte subsets in the tumor microenvironment with follicular lymphoma
Mingxia Zhu, Wenli Wan. Department of Hematology, Lymphoma Research Center, Peking University Third Hospital, Beijing China

118

A Phase I Study Evaluating the Pharmacodynamics of a Novel Recombinant Type II FcγR Agonist (NVG-2089) in Healthy Volunteers
Greg P. Coffey¹, Jessica M. Bright¹, Pamela B. Conley¹, Alexis N. Conway². ¹Nuvig Therapeutics, Inc., Menlo Park, CA, USA.²ACM Global Laboratories, Rochester, NY, USA

119

Plagued by Autofluorescence: Case Report and Discussion of a Peculiar Peripheral Blood Monoclonal B-cell Population
Renee E Dumm, Aditya S Patel, Isaac E McCool. Brooke Army Medical Center, San Antonio, TX, USA

120

T-SNE plots can be used to improve detection of abnormal immature B-cells in BLL MRD analysis
Jessica M Hughes, Lorinda A Soma, Joo Y Song, Winston Y Lee. City of Hope National Medical Center, Duarte, CA, USA

121

Utility of Flow Cytometric Analysis in Diagnosis, Prediction of Molecular Genetic Abnormalities and Prognosis in CD23-Positive Plasma Cell Leukemias: A Case Report and Literature Review
Nobuyuki Mukai¹, Isaac McCool². ¹National Defense Medical College Hospital, Tokorozawa, Japan.²Brooke Army Medical Center, San Antonio, TX, USA

122

Use of CD32, CD44, and CD71 to Differentiate B-cell Lymphoma Subtypes by Flow Cytometry
Afshin Shameli¹, Jason E Love¹, Kikkeri N Naresh^{1, 2}, Sindhu Cherian¹, Jonathan R Fromm¹. ¹Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, WA, USA.²Pathology Program, Translational Science & Therapeutics Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

123

Experience with flow cytometric evaluation of TRBC1 expression in the assessment of T-cell clonality in a pediatric institution.
Miguel Francoise Ventura¹, Sarah Bowman¹, Robert Lorsbach^{1, 2}. ¹Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.²University of Cincinnati, Cincinnati, OH, USA

124

High-sensitivity NGS (ClonoSEQ) and Flow Cytometry are Complementary for MM MRD Assessment, with ClonoSEQ Detecting Lower-level MRD and Stratifying Event-free Survival
Cuiping Zhang², Joe Tario¹, You-Wen Qian¹. ¹Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.²University of Missouri, Columbia, MO, USA

125

New IR820 and IR870 infrared antibody conjugates enable low noise lineage gating in conventional and spectral flow cytometry
Salima Anastasakis¹, Rebecca Pearson², Brandy Pinckney², Sharon Sen², Samantha Bauchiero², Michael Kapinsky³. ¹Beckman Coulter Canada, LP, Mississauga, ON, Canada.²Beckman Coulter Life Sciences, Indianapolis, IN, USA.³Beckman Coulter GmbH, Krefeld, Germany

127

Performance assessment of a Novel Hematology Flow Cell Imaging Technology for the Identification of Neoplastic Cells of Hematopoietic Origin
Andreea M. Foghis¹, Bart J. Wanders², Rob Monroe³, Logan Hallen³, Lilian Borisov³, Todd Druley³, Brittany Roemmich¹, Mark Watson¹, Christopher Farnsworth¹, Cara Lunn Shirai¹. ¹Department of Pathology and Immunology, Washington University, Saint Louis, MO, USA.²Beckman Coulter, Inc, Brea, CA, USA.³Danaher Diagnostics, Washington, DC, USA

128

Reducing Operator-Dependent Variability in Immunophenotyping Through Automated Antibody Panel Preparation
Basile Siewe¹, Amira Amilia Amiruddin¹, Kavita Mathi², Sashigala Ponnalagu¹, Jing Xuan Koh¹, Zi Xu Chai¹, Holden Maecker², Chyan Ying Ke¹, Namyong Kim². ¹Curiox Biosystems, Woburn, MA, USA.²Human Immunomonitoring Center, Stanford University, Palo Alto, CA, USA

129

Standardized immunophenotyping of UK NEQAS B-CLL samples on the BD FACSLyric™ Flow Cytometer using BD OneFlow™ LST and B-CLPD T1 Panels
Yang Zeng, Farzad Oreizy, Angela Chen. BD Biosciences, Milpitas, CA, USA

130

Evaluating Beads Performance on the CellMek SPS Instrument
Xizi (Daisy) Dai, Xue Wen, Jessica Ashbaugh, Juan Fernandez De Castro, Ernesto Staroswiecki. Beckman Coulter Life Sciences, Miami, FL, USA

131

Cloud-Based Flow Cytometry Reagent Tracking in a GCP Regulated Laboratory
Taryn Mockus¹, Ciara Gimblet-Ochieng¹, Ekambaram Ganapathy¹, Esther Perez Garcia², Michael Naughton², Ruth Barnard², Veronica Nash¹, Vilma Decman¹. ¹Cellular Biomarkers, Biomarker and Bioanalytical Platforms, GSK, Collegeville, PA, USA.²Cellular Biomarkers, Biomarker and Bioanalytical Platforms, GSK, Stevenage United Kingdom

132

Reliable immunophenotyping of UK NEQAS PCM MRD samples using dried MM-MRD reagents, BD FACSLyric™ Flow Cytometer, and BD Infinicyt™ Software
Yang Zeng, Farzad Oreizy, Angela Chen. BD Biosciences, Milpitas, CA, USA

133

Flow Cytometry in a Routine Diagnostic Workflow of Pediatric Lymphomas
Felipe Furtado^{1, 2}, Stefany Leal¹, Roberia Pontes¹, Pedro Pinto¹, Elaine Costa³, Ricardo Camargo¹. ¹Hospital da CrianÃ§a de BrasÃlia, BrasÃlia, Brazil.²Sabin Medicina DiagnÃ³stica, BrasÃlia, Brazil.³Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

134

A Dried 20-Color Panel for Cross-Instrument Standardization in Spectral and Imaging Cytometry
Rodrigo Pestana Lopes, Woodrow Lomas, April Apostol, Scott Souza, Chandrima Ghosh, Aaron Middlebrook. BD Biosciences, Milpitas, CA, USA

135

Challenges In Standardising A G6PD Deficiency Screening Method Based On Flow Cytometry
Shreyam Acharya¹, Jasmita Dass¹, Shailja Singh², Ashok Jaiswal ¹, Richa Chauhan¹, Mukul Aggarwal¹, Shreeja Biswas², Swati Garg², Ganesh Kumar V¹, Tulika Seth¹, Anu Thukral¹, Vishal Gupta¹, Ravi Ranjan ¹, Rishi Dhawan¹, Pradeep Kumar¹, Manoranjan Mahapatra¹. ¹All India Institute of Medical Sciences, New Delhi, India.²Jawaharlal Nehru University, New Delhi, India

136

Integrative Analysis of Full-Length TCR Sequencing and CITE-Seq to Uncover Pathogenic T Cell Signatures in Psoriasis
Ali Rezvan¹, Cynthia Sakosfky¹, Gracia Genero¹, Lorena Hidalgo², Hye-Won Song², Aruna Ayer¹, Stephanie Widmann², Gisele Baracho². ¹BD Biosciencies, Milpitas, CA, USA.²BD Bisociencies, San Diego, CA, USA

137

B/T Mixed-Phenotype Acute Leukemia, Rare Types
Melissa Whittaker, Karry Muzzey, Daniel Dees, Karen Ferreira. Mass General Brigham, Boston, MA, USA

138

Simultaneous detection of aggressive NK cell leukemia and reactive KIR-restricted adaptive NK cells.
Aaron J Wilk, Jean Oak. Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA

139

A Rare Convergence: First Report of Synchronous Hairy Cell Leukemia and Leukemic Non-Nodal Mantle Cell Lymphoma
Dina S Soliman^{1, 2, 3}, Omnya Abdalla¹, Suhair Hassan¹. ¹Hamad medical corporation, Doha, Qatar.²National Cancer Institute, Cairo University, Cairo, Egypt.³Weill Cornell Medicine-Qatar, Doha, Qatar

140

A Challenging Case of Bilineal Mixed Phenotypic Acute Leukemia
Zubaidah Al-Jumaili¹, Reshma Kulkarni², Vladimir Miltchev¹, Lucas McGowan¹, Natalia .Golardi¹. ¹Baylor College of Medicine, Houston, TX, USA.²Harris health Ben Taub Hospital, Houston, TX, USA

141

Dual Expression of CD19 and CD7 in Myeloid Blasts: An Immunophenotypic Clue to Underlying MLLT10 Rearrangement?
Shreyam Acharya, Jasmita Dass, Richa Chauhan, Vinu Balraam KV, Ganesh KV, Mukul Aggarwal, Pradeep Kumar, Rishi Dhawan, Tulika Seth, Manoranjan Mahapatra. All India Institute of Medical Sciences, Delhi, India

7:30 - 8:30 PM
Meeting

WOMEN IN CYTOMETRY

Commonwealth BCD

7:30

Introductions
Fabienne Lucas.

7:45

When We Work Together, We Succeed Together
Melanie O'Donahue.

Program

Program

Course & Meeting Program

Please find the 2025 Meeting & Course Program below. The course will take place on Friday and finish at noon (12pm) on Sunday. The meeting will then start on Sunday afternoon and finish on Tuesday. All program times are listed in the Eastern Time zone.

Sunday, September 28th

Please find the 2025 Meeting & Course Program below.

The course will take place on Friday and finish at noon (12pm) on Sunday.
The meeting will then start on Sunday afternoon and finish on Tuesday.

All program times are listed in the Eastern Time zone.