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| Case Study Interpretation (CSI) |
Clinical History: 51 year-old male with leukopenia, anemia and thrombocytopenia.
| CBC parameter |
Result |
Units |
Reference Range (Male/Female) |
| WBC |
1.9 |
x109/L |
(3.8 - 10.6) |
| RBC |
3.74 |
x1012/L |
(4.13 - 5.57 / 3.73 - 4.89) |
| Hgb |
13.0 |
g/dl |
(12.9 - 16.9 / 11.6 - 14.6) |
| Hct |
38.3 |
% |
(38.0 - 48.8 / 34.1 - 43.3) |
| MCV |
102.3 |
fl |
(82.6 - 97.4) |
| MCH |
34.9 |
pg |
(27.8 - 33.4) |
| MCHC |
34.1 |
gm/dl |
(32.7 - 35.5) |
| RDW |
14.2 |
% |
(11.8 - 15.2) |
| PLT |
103 |
x109/L |
(156 - 369 ) |
| WBC Differential |
% |
Absolute |
Reference Range (Absolute) |
| Polys |
26 |
0.49 x109/L |
(2.24 7.68 ) |
| Bands |
2 |
0.04 x109/L |
(0.10 - 0.80 ) |
| Lymphocytes |
68 |
1.29 x109/L |
(0.80 - 3.65 ) |
| Atypical lymphs |
3 |
0.06 x109/L |
|
| Monocytes |
1 |
0.02 x109/L |
(0.30 - 0.90 ) |
Bone marrow aspirate and biopsy were received for evaluation.
Flow cytometric immunophenotyping was performed on a portion of the bone marrow aspirate and the results from selected 4-color tubes are provided for review:
Acquired FACS Canto II (FITC / PE / PerCP-Cy5.5 / APC)
• Tube 1: CD14 / CD13&33 / CD45 / CD34
• Tube 2: CD38 / CD22 / CD20 / CD10
• Tube 3: Kappa / Lambda / CD19 / CD5
• Tube 4: CD103 / CD11c / CD20 / CD25
Download FCS 2.0 files (ZIP format)
Download PDF files with example analysis (PDF format):
Tube 1 Analysis
Tube 2 Analysis
Tube 3 Analysis
Tube 4 Analysis
Download/View Case discussion (Powerpoint format)
James T. Edinger, MD and Fiona E. Craig, MD
Division of Hematopathology, Department of Pathology,
University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
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Would You Like to Contribute a Case Study?
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You will need to provide:
1. A brief case history including demographic data, specimen type, relevant clinical history, and indication for study.
2. A list of the tubes selected for review with the antibodies and fluorochromes used.
3. Selected anonomized list mode files to post on the ICCS website. List mode files should be labeled with a key detailing antibodies and fluorochromes used, the format in which files were collected (FCS2.0 or FCS3.0, eg) and the software used for collection.
4. PDF files for each tube selected, showing an example of what a correct and complete analysis should look like.
5. A Powerpoint presentation summarizing your case, to include a description of a recommended analysis strategy, the flow cytometric pertinent findings, and their significance.
6. A passport-style photo of each author.
7. A signed web consent form.
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